Protein-L Cys

Protein L Cys Recombinant
Cat. No.
BT9786
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Recombinant Protein-L produced in E.Coli is a single non-glycosylated polypeptide chain fused with a Cys at C-terminus. Protein-L is comprised of 5 IgG-binding regions of protein L (B1-B2-B3-B4-B5) containing 366 amino acids in total and having a molecular mass of 40.6kDa, however, it migrates with an apparent molecular mass of 46kDa on SDS-PAGE. Cell wall binding region, cell membrane binding region and albumin binding region have been eliminated from the recombinant Protein-L to guarantee the maximum specific IgG binding.

Product Specs

Introduction
Recombinant Protein L, composed of five kappa-binding domains, exhibits the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This characteristic enables Protein L to bind a wider range of immunoglobulin classes and subclasses compared to other antibody-binding proteins, making it ideal for purifying polyclonal or monoclonal IgG antibodies. Protein L demonstrates binding affinity for IgG derived from various species, including humans, mice, rats, and pigs.
Description
Produced in E. coli, Recombinant Protein-L is a single, non-glycosylated polypeptide chain with a C-terminal Cys fusion. Comprising five IgG-binding regions (B1-B2-B3-B4-B5) of protein L, it encompasses 366 amino acids with a molecular mass of 40.6 kDa. However, it exhibits an apparent molecular mass of 46 kDa on SDS-PAGE. To ensure maximum specific IgG binding, cell wall binding, cell membrane binding, and albumin binding regions have been removed from the recombinant Protein-L.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Protein-L was lyophilized without the addition of any other substances.
Solubility
For reconstitution of the lyophilized Protein-L, sterile 18M-cm H2O is recommended at a concentration not lower than 0.1mg/ml. Further dilutions can be made using other aqueous solutions.
Stability
Lyophilized Protein-L remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, Protein-L should be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is advised. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage.
Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 96.0% as determined by:
(a) SEC-HPLC analysis.
(b) SDS-PAGE analysis.
Source
Escherichia Coli.
Amino Acid Sequence
KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAGC.

Product Science Overview

Introduction

Protein L Cys Recombinant is a genetically engineered protein derived from the bacterium Peptostreptococcus magnus. This recombinant protein is designed to bind immunoglobulin (Ig) molecules through their kappa light chains, without interfering with the antigen-binding sites of the antibodies. This unique binding property makes Protein L Cys Recombinant an invaluable tool in the purification and detection of antibodies, particularly those that do not bind well to Protein A or Protein G.

Structure and Composition

Recombinant Protein L contains five IgG-binding domains, each capable of binding to the kappa light chains of antibodies. The recombinant version is typically produced in Escherichia coli and is a single non-glycosylated polypeptide chain. It has a molecular weight of approximately 40.6 kDa, but it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE due to its structure .

Preparation Methods

The production of Protein L Cys Recombinant involves the following steps:

  1. Gene Cloning: The gene encoding Protein L is cloned into an expression vector suitable for E. coli.
  2. Expression: The recombinant E. coli cells are cultured, and the expression of Protein L is induced.
  3. Purification: The protein is purified from the bacterial lysate using affinity chromatography, typically involving an IgG column to capture the Protein L.
  4. Lyophilization: The purified protein is then lyophilized (freeze-dried) to ensure stability and ease of storage .
Chemical Reactions and Analysis

Protein L Cys Recombinant binds to the kappa light chains of antibodies through non-covalent interactions. This binding does not interfere with the antigen-binding sites of the antibodies, allowing for the purification of a wide range of Ig classes and subclasses. The protein’s purity is typically assessed using SDS-PAGE and SEC-HPLC analyses, ensuring a purity level of over 96% .

Regulatory Mechanisms

The recombinant Protein L is designed to be free from endotoxins, with levels less than 0.1 EU/μg as determined by the Limulus Amebocyte Lysate (LAL) method. This ensures that the protein is suitable for various research applications without causing adverse reactions .

Applications

Protein L Cys Recombinant is widely used in:

  • Antibody Purification: Ideal for purifying polyclonal and monoclonal antibodies, especially those that do not bind well to Protein A or Protein G.
  • Immunoassays: Used in various immunoassays to detect and quantify antibodies.
  • Research: Employed in research studies involving antibody interactions and mechanisms .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Cys-Protein-L
Cys-Protein L Recombinant
Cat. No.
BT8941
Source
Escherichia Coli.
Protein-L
Protein L Recombinant
Cat. No.
BT9705
Source
Escherichia Coli.
Protein-L Cys, His
Protein-L Cys Recombinant, His Tag
Cat. No.
BT9873
Source
Escherichia Coli.
Protein-L, His
Protein L Recombinant, His Tag
Cat. No.
BT9961
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.