Protein-L

Protein L Recombinant
Cat. No.
BT9705
Source
Escherichia Coli.
Synonyms
SPL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Protein-L Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 365 amino acids and having a molecular mass of 40.5 kDa but it migrates with an apparent molecular mass of 45 kDa in SDS-PAGE.
The Protein-L is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Recombinant Protein L, a genetically engineered protein, possesses five IgG-binding regions. It uniquely binds through kappa light chain interactions without hindering the antibody's antigen-binding site. The removal of cell wall binding, cell membrane binding, and albumin binding regions guarantees maximum specific IgG binding. This makes recombinant Protein L ideal for purifying polyclonal or monoclonal IgG antibodies from various species, including human, mouse, rat, and pig.
Description
Produced in E. coli, recombinant Protein-L is a single, non-glycosylated polypeptide chain comprising 365 amino acids. It has a molecular mass of 40.5 kDa but exhibits an apparent molecular mass of 45 kDa in SDS-PAGE analysis.
Protein-L is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Protein-L is lyophilized without any additives.
Solubility
For reconstitution, dissolve the lyophilized Protein-L in sterile water or saline at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
While lyophilized Protein-L remains stable at room temperature for up to 3 weeks, it is recommended to store it desiccated below -18°C. After reconstitution, Protein-L should be stored at 4°C for 2-7 days. For long-term storage, freeze below -18°C, ideally with a carrier protein like 0.1% HSA or BSA.
Avoid repeated freeze-thaw cycles.
Purity

The purity is determined to be greater than 97.0% using the following methods:
(a) SEC-HPLC analysis.
(b) SDS-PAGE analysis.

Synonyms
SPL.
Source
Escherichia Coli.
Amino Acid Sequence
KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE
EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAG

Product Science Overview

Recombinant Production

Recombinant Protein L is produced using Escherichia coli expression systems . The recombinant form is genetically engineered to remove regions that bind to cell walls, cell membranes, and albumin, enhancing its specificity for immunoglobulins . This recombinant production ensures a high yield and purity of Protein L, making it suitable for various research and biotechnological applications.

Applications

Protein L is widely used in the purification of monoclonal antibodies, especially those with kappa light chains . Its ability to bind to a wide range of immunoglobulin classes makes it a valuable tool in antibody purification processes. Additionally, Protein L is used in flow cytometry and other immunoassays to detect and quantify kappa light chain-containing immunoglobulins .

Advantages
  1. Broad Specificity: Protein L can bind to multiple immunoglobulin classes and subclasses, including Fab fragments and scFvs .
  2. High Affinity: It has a high affinity for kappa light chains, making it effective in antibody purification .
  3. Recombinant Production: The recombinant form ensures high purity and yield, reducing the risk of contamination .

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