Cys-Protein-L

Cys-Protein L Recombinant is a single, non-glycosylated polypeptide chain containing 366 amino acids, with a molecular weight of approximately 40.6 kDa . However, it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE due to its specific structure . The protein includes a cysteine (Cys) residue at either the N-terminus or C-terminus, depending on the specific variant .

One of the most notable features of Cys-Protein L Recombinant is its ability to bind to the kappa light chains of immunoglobulins without interfering with the antigen-binding sites . This unique property allows it to bind a wide range of Ig classes and subclasses, making it highly versatile for antibody purification .

Cys-Protein L Recombinant is ideal for the purification of polyclonal or monoclonal IgG antibodies from various species, including humans, mice, rats, and pigs . It is particularly useful in applications where the presence of albumin or other proteins might interfere with the purification process, as the cell wall binding region, cell membrane binding region, and albumin binding region have been removed from the recombinant protein .

The recombinant protein is produced in Escherichia coli and is available in a lyophilized (freeze-dried) form . It is highly pure, with a purity level greater than 95% as determined by SDS-PAGE analysis . The endotoxin level is less than 0.1 EU/μg, ensuring that it is suitable for sensitive applications .

Cys-Protein L Recombinant is stable for 12 months from the date of receipt when stored at -20 to -70°C . After reconstitution, it remains stable for one month at 2 to 8°C under sterile conditions and for three months at -20 to -70°C under sterile conditions . It is important to avoid repeated freeze-thaw cycles to maintain its stability .

The lyophilized protein can be reconstituted in distilled water or saline . This makes it easy to prepare for use in various experimental protocols.