Protein-L Cys, His
Description
Product Specs
Product Science Overview
Recombinant Protein-L is produced in Escherichia coli (E. coli) and is a single non-glycosylated polypeptide chain. It is fused with a 6×His tag at the N-terminus and a cysteine (Cys) residue at the C-terminus . The protein comprises five IgG-binding regions (B1-B2-B3-B4-B5) of Protein L, containing a total of 373 amino acids and having a molecular mass of approximately 41.6 kDa. However, it migrates with an apparent molecular mass of 46 kDa on SDS-PAGE .
Protein L has the exceptional ability to bind a wide range of Ig classes and subclasses from various species, including humans, mice, rats, and pigs . This broad binding capability is due to its interaction with the kappa light chains of antibodies, which does not hinder the antigen-binding site. This property makes Protein L an ideal tool for the purification of polyclonal or monoclonal IgG antibodies .
The recombinant Protein-L Cys, His Tag is widely used in:
- Antibody Purification: Due to its ability to bind a broad range of Ig classes and subclasses, it is extensively used for purifying antibodies from different species.
- Immunoassays: It is used in various immunoassays for detecting and quantifying antibodies.
- Research: It serves as a valuable tool in research involving antibody-antigen interactions and other immunological studies.
The recombinant Protein-L is produced in E. coli and is available as a sterile filtered, white lyophilized (freeze-dried) powder . It is formulated without any additives and has a purity greater than 96% as determined by SDS-PAGE . The protein is stable at room temperature for up to three weeks when lyophilized but should be stored desiccated below -18°C for long-term storage .
For use, the lyophilized Protein-L should be reconstituted in sterile water or saline to a concentration of not less than 0.1 mg/ml . After reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage. It is recommended to add a carrier protein (0.1% HSA or BSA) to prevent freeze-thaw cycles .
