Protein-A/G Cys

Protein A/G Cys Recombinant
Cat. No.
BT9382
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Protein-A/G Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain fused with a Cys at C-terminus. Protein-A/G is comprised of 5 IgG-binding regions of protein A (E-D-A-B-C) and 2 of protein G (C1-C3) containing 430 amino acids in total and having a molecular mass of 47.8kDa. Cell wall binding region, cell membrane binding region and albumin binding region have been eliminated from the recombinant Protein A/G to guarantee the maximum specific IgG binding.

Product Specs

Introduction
Engineered by combining specific domains from Protein A and Protein G, recombinant Protein A/G boasts an enhanced IgG binding capacity. This chimeric protein incorporates seven IgG-binding domains: EDABC from Staphylococcus aureus Protein A and C1C3 from Streptococcus Protein G. This design enables broader specificity compared to its parental proteins, making it highly effective for purifying both monoclonal and polyclonal IgG antibodies from various species, including human, mouse, rat, cow, goat, sheep, horse, rabbit, guinea pig, pig, dog, and cat. Notably, it exhibits affinity for human IgG subtypes 1, 2, 3, and 4, mouse IgG subtypes 2a, 2b, and 3, and rat IgG subtypes 2a and 2c.
Description
Recombinant Protein A/G, produced in E. coli, is a non-glycosylated polypeptide chain with a C-terminal cysteine residue. This engineered protein consists of five IgG-binding regions derived from Protein A (E-D-A-B-C) and two from Protein G (C1-C3), totaling 430 amino acids and exhibiting a molecular weight of 47.8 kDa. To maximize specific IgG binding, the recombinant Protein A/G lacks the cell wall binding region, cell membrane binding region, and albumin binding region.
Physical Appearance
White, lyophilized powder, sterilized by filtration.
Formulation
Protein-A/G is lyophilized without the addition of any excipients.
Solubility
For reconstitution, it is advised to dissolve the lyophilized Protein-A/G in sterile 18 MΩ·cm water to a minimum concentration of 0.1 mg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized Protein-A/G remains stable at room temperature for up to three weeks. However, for extended storage, it should be stored in a dry environment below -18°C. After reconstitution, Protein-A/G can be stored at 4°C for 2-7 days. For long-term storage, freezing at -18°C is recommended, ideally with the addition of a carrier protein like 0.1% HSA or BSA. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of this product exceeds 96.0%, as determined by High-Performance Liquid Chromatography (HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Source
Escherichia Coli.
Amino Acid Sequence
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEC.

Product Science Overview

Composition and Structure

Protein A/G Cys Recombinant is composed of seven IgG-binding domains:

  • Protein A: Derived from Staphylococcus aureus, it includes segments E, D, A, B, and C.
  • Protein G: Derived from Streptococcus, it includes segments C1 and C3.

The recombinant protein is a single non-glycosylated polypeptide chain containing 430 amino acids and has a molecular mass of approximately 47.8 kDa . It is produced in Escherichia coli and is fused with a cysteine (Cys) at the C-terminus to facilitate its use in various applications.

Binding Capacity

Protein A/G Cys Recombinant has a broader binding capacity compared to individual Protein A or Protein G. It can bind to various human, mouse, and rat IgG subclasses, including:

  • Human IgG1, IgG2, IgG3, IgG4
  • Mouse IgG2a, IgG2b, IgG3
  • Rat IgG2a, IgG2c

Additionally, it binds to total IgG from other species such as cow, goat, sheep, horse, rabbit, guinea pig, pig, dog, and cat .

Applications

The primary application of Protein A/G Cys Recombinant is in the purification of monoclonal or polyclonal IgG antibodies. Its broad binding capacity makes it ideal for use in various immunoassays, including:

  • Immunoprecipitation
  • Western blotting
  • ELISA (Enzyme-Linked Immunosorbent Assay)
Stability and Storage

Protein A/G Cys Recombinant is typically provided as a sterile, lyophilized (freeze-dried) powder. For optimal stability, it should be stored desiccated below -18°C. Upon reconstitution, it can be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage. To prevent degradation, it is recommended to avoid freeze-thaw cycles and to add a carrier protein (e.g., 0.1% HSA or BSA) for long-term storage .

Purity and Quality Control

The purity of Protein A/G Cys Recombinant is greater than 96%, as determined by HPLC and SDS-PAGE analysis . It is also tested for endotoxin levels, ensuring it is suitable for laboratory research use.

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