Cys-Protein-G
Description
Cys-Protein G Recombinant produced in E.Coli, is a single non-glycosylated polypeptide chain containing 201 amino acids and having a cys on N-terminal. Cys-Protein G has a predicted molecular mass of approximately 21.9kDa but it migrates with an apparent molecular mass of 40kDa in SDS-PAGE. The Cys-Protein G is purified by proprietary chromatographic techniques.
Product Specs
CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E.
The recombinant Protein G is a genetically engineered protein contains 3 IgG-binding regions of protein G.
Product Science Overview
Cys-Protein G Recombinant consists of a single non-glycosylated polypeptide chain containing 201 amino acids . It includes three IgG-binding regions of protein G, with a cysteine (Cys) residue added at the N-terminus . The addition of the cysteine residue allows for site-specific conjugation, which can be useful in various biochemical assays and applications.
The recombinant protein is produced in Escherichia coli (E. coli) and has a theoretical molecular weight of approximately 21.9 kDa . However, it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE due to its specific structure and properties .
Protein G is known for its high affinity binding to the Fc region of immunoglobulins (IgG) from various species, including human, mouse, rat, cow, goat, sheep, horse, and rabbit . This binding capability makes Cys-Protein G Recombinant particularly valuable for the purification of polyclonal and monoclonal IgG antibodies .
The recombinant version has been engineered to remove the cell wall binding region, cell membrane binding region, and albumin binding region, ensuring maximum specific IgG binding . This enhances its specificity and efficiency in binding to IgG subclasses, such as human IgG1, IgG2, IgG3, and IgG4, as well as mouse IgG2a, IgG2b, and IgG3 .
Cys-Protein G Recombinant is highly purified, with a purity greater than 95% as determined by SDS-PAGE and HPLC . The endotoxin level is less than 0.1 EU/µg, as determined by the Limulus Amebocyte Lysate (LAL) method .
For storage, the protein is typically lyophilized from an additive-free solution and should be stored at -20°C to -70°C . After reconstitution, it can be stored at 2°C to 8°C for up to one month or at -20°C to -70°C for long-term storage . It is important to avoid repeated freeze-thaw cycles to maintain its stability and functionality .
