Protein-G His
Description
The Protein G His Tag is purified by proprietary chromatographic techniques.
Product Specs
2. Does not bind to human IgM, IgD and IgA.
Product Science Overview
Recombinant Protein G is produced using gene cloning and expression techniques. The gene encoding Protein G is inserted into a vector, which is then introduced into a host system, such as Escherichia coli (E. coli). The host cells express the recombinant protein, which can then be purified for various applications.
The His tag (histidine tag) is a string of six to nine histidine residues added to either the N- or C-terminus of a protein. This tag facilitates the purification and detection of recombinant proteins. The His tag binds to immobilized metal ions, such as nickel, cobalt, and copper, under specific buffer conditions. This binding allows for the efficient purification of His-tagged proteins using immobilized metal affinity chromatography (IMAC) .
Recombinant Protein G with a His tag is widely used in research and clinical laboratories for the following purposes:
- Antibody Purification: Protein G binds to the Fc region of IgG antibodies from various species, making it an excellent tool for purifying antibodies from complex mixtures.
- Immunoprecipitation: The His tag allows for easy purification and detection of Protein G, which can then be used to immunoprecipitate antibodies and their target antigens.
- Diagnostic Assays: Protein G is used in various diagnostic assays to detect and quantify antibodies in biological samples.
The production of recombinant Protein G involves several steps:
- Gene Cloning: The gene encoding Protein G is cloned into an expression vector along with the His tag sequence.
- Expression: The vector is introduced into a host system, such as E. coli, which expresses the recombinant protein.
- Purification: The His-tagged Protein G is purified using IMAC, where the histidine residues bind to immobilized metal ions, allowing for the selective purification of the recombinant protein .
Recombinant Protein G with a His tag is typically lyophilized for long-term storage. It should be stored at -20°C or lower to maintain its stability. The lyophilized product can be reconstituted according to the manufacturer’s instructions and should be used under sterile conditions to prevent contamination .
