Procalcitonin Rhesus
Procalcitonin Rhesus Recombinant
Cat. No.
BT18284
Source
Escherichia Coli.
Synonyms
Calcitonin.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Procalcitonin Rhesus Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ala26-Asn140) containing 125 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 14kDa.
Product Specs
Introduction
Procalcitonin is a peptide hormone primarily produced by the thyroid's C cells and specific endocrine cells within the lungs. Typically, procalcitonin undergoes immediate cleavage into three distinct fragments: an N-terminal residue, calcitonin, and katacalcin. However, levels of uncleaved procalcitonin increase significantly in response to bacterial infections, trauma, or shock.
Description
Recombinant Rhesus Procalcitonin, produced in E. coli, is a single, non-glycosylated polypeptide chain (Ala26-Asn140) consisting of 125 amino acids. This includes a 10 amino acid His tag located at the N-terminus. The calculated molecular mass is 14 kDa.
Physical Appearance
White lyophilized (freeze-dried) powder, filtered for appearance.
Formulation
Procalcitonin undergoes a 0.4 μm filtration process before being lyophilized from a 0.5 mg/mL solution. This solution comprises 20 mM Tris buffer and 50 mM NaCl at a pH of 7.5.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet aiming for a concentration of approximately 0.5 mg/mL. Allow sufficient time for complete dissolution. Note: This Procalcitonin product is not sterile. Prior to cell culture use, it is essential to filter the solution through an appropriate sterile filter.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. While the reconstituted protein can be stored at 4°C for a limited period, it's essential to note that it exhibits stability for up to two weeks at this temperature.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Calcitonin.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASAPFRSALESS PDPATLSEEE ARLLLAALVQ DYVQMKASEL EQEQETEGSS LDSPRSKRCG NLSTCMLGTY TQDFNKFHTF PQTAIGVGAP GKKRDMSSDL ERNRRRYVSM PQDAN.
Product Science Overview
Production and Purification
The production of recombinant procalcitonin involves several steps:
- Gene Cloning: The gene encoding procalcitonin is isolated and inserted into a plasmid vector, which is then introduced into a host organism like E. coli.
- Expression: The host organism is cultured under conditions that promote the expression of the procalcitonin gene, leading to the production of the recombinant protein.
- Purification: The recombinant procalcitonin is purified from the host cells using techniques such as affinity chromatography, which ensures a high degree of purity (greater than 97% as determined by SDS-PAGE) .
Clinical Significance
Procalcitonin is a significant biomarker used in the medical field to diagnose bacterial infections and sepsis. Its levels in the blood increase significantly in response to systemic inflammation caused by bacterial infections . This makes it a valuable tool for guiding antibiotic therapy and predicting patient outcomes in various clinical settings .
Applications
Recombinant procalcitonin is used in various research and clinical applications, including:
- Diagnostic Assays: It is used in assays to measure procalcitonin levels in patient samples, aiding in the diagnosis of bacterial infections and sepsis .
- Therapeutic Research: It is used in research to develop new therapeutic strategies for managing bacterial infections and sepsis.
- Biological Studies: It is used in studies to understand the biological functions and mechanisms of procalcitonin and its role in the immune response .
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