Procalcitonin Human, His
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
The PCT is purified by standard chromatographic techniques.
Product Specs
Product Science Overview
Recombinant human procalcitonin with a His tag is typically produced in Escherichia coli (E. coli) expression systems. The recombinant protein consists of a single, non-glycosylated polypeptide chain containing 114 amino acids, with a molecular mass of approximately 17.13 kDa . The His tag, which is a sequence of histidine residues, is added to the N-terminus of the protein to facilitate purification using affinity chromatography .
The recombinant procalcitonin is purified using standard chromatographic techniques to achieve high purity levels, often greater than 95% as determined by SDS-PAGE under reducing conditions . This high purity makes it suitable for various applications, including:
- Western Blotting: Used as a positive control to detect procalcitonin in samples.
- ELISA: Employed in sandwich ELISA assays to quantify procalcitonin levels in biological samples.
- Research: Utilized in studies investigating the role of procalcitonin in calcium regulation and its potential as a biomarker for bacterial infections .
Procalcitonin is primarily known for its role in calcium regulation. Calcitonin, one of the hormones derived from procalcitonin, helps lower blood calcium levels by inhibiting osteoclast activity and promoting calcium deposition in bones . Additionally, calcitonin gene-related peptide functions as a vasodilator, while katacalcin is involved in lowering calcium levels .
In clinical settings, procalcitonin is used as a biomarker for bacterial infections and sepsis. Elevated levels of procalcitonin in the blood can indicate a systemic bacterial infection, making it a valuable tool for diagnosing and monitoring sepsis .
