Procalcitonin Mouse

Procalcitonin is encoded by the CALCA gene. The mature form of procalcitonin is a 116 amino acid protein, which is subsequently cleaved into three parts:

• A 57 amino acid pro-region
• A 32 amino acid calcitonin peptide
• A 21 amino acid katacalcin peptide

In healthy individuals, procalcitonin is expressed by thyroid C cells and is promptly converted to calcitonin. Calcitonin plays a crucial role in regulating calcium levels in the blood by inhibiting osteoclast activity and bone resorption .

Recombinant procalcitonin, including mouse recombinant procalcitonin, is typically produced using Escherichia coli (E. coli) expression systems. The recombinant protein is a single, non-glycosylated polypeptide chain containing 116 amino acids . The production process involves:

1. Gene Cloning: The CALCA gene encoding procalcitonin is cloned into an expression vector.
2. Transformation: The vector is introduced into E. coli cells, which are then cultured to express the recombinant protein.
3. Purification: The recombinant procalcitonin is purified using techniques such as SDS-PAGE to ensure a purity of over 95% .

Procalcitonin levels in the blood can rise significantly in response to bacterial infections, trauma, or shock. This makes it a valuable biomarker for diagnosing bacterial infections and sepsis. Elevated procalcitonin levels can help differentiate bacterial infections from other causes of inflammation .

Recombinant procalcitonin is used extensively in research to study its role in various physiological and pathological processes. It is also used in the development of diagnostic assays and therapeutic interventions.

Recombinant procalcitonin is typically lyophilized and should be stored at -20°C to -80°C to maintain its stability. It is reconstituted in sterile water or phosphate-buffered saline (PBS) before use .