Procalcitonin Porcine
Description
Product Specs
Product Science Overview
Procalcitonin is a peptide precursor of the hormone calcitonin, which is primarily produced by the thyroid gland. It is composed of 116 amino acids and is encoded by the CALC-1 gene. Under normal physiological conditions, procalcitonin levels in the blood are very low. However, during systemic inflammation, particularly due to bacterial infections, the levels of procalcitonin can rise significantly, making it a valuable biomarker for diagnosing sepsis and other bacterial infections .
Recombinant Procalcitonin refers to procalcitonin that is produced using recombinant DNA technology. This involves inserting the gene that encodes procalcitonin into a host organism, such as bacteria or yeast, which then produces the protein. This method allows for the production of large quantities of procalcitonin for research and clinical use .
Porcine Recombinant Procalcitonin specifically refers to procalcitonin that is derived from pigs. Pigs are often used in biomedical research due to their physiological similarities to humans. The recombinant form of porcine procalcitonin is produced by inserting the porcine procalcitonin gene into a host organism, which then expresses the protein. This recombinant protein can be used in various research applications, including the study of sepsis and other inflammatory conditions .
Procalcitonin is a crucial biomarker in the medical field. Its levels in the blood increase significantly in response to bacterial infections, making it a valuable tool for diagnosing sepsis. Sepsis is a life-threatening condition caused by the body’s response to an infection, leading to tissue damage, organ failure, and potentially death. Early diagnosis and treatment are critical for improving patient outcomes, and procalcitonin levels can help guide clinical decisions regarding the use of antibiotics and other treatments .
The production of recombinant procalcitonin involves several steps:
- Gene Cloning: The gene encoding procalcitonin is isolated and inserted into a plasmid vector.
- Transformation: The plasmid vector is introduced into a host organism, such as Escherichia coli.
- Expression: The host organism expresses the procalcitonin protein.
- Purification: The recombinant procalcitonin is purified from the host organism using various chromatographic techniques.
Recombinant procalcitonin is used in various research and clinical applications, including:
- Diagnostic Assays: Procalcitonin levels are measured in blood samples to diagnose bacterial infections and sepsis.
- Research Studies: Recombinant procalcitonin is used in studies to understand the mechanisms of sepsis and other inflammatory conditions.
- Therapeutic Development: Research on procalcitonin can lead to the development of new therapies for sepsis and other diseases .
