PRL R Rainbow Trout
Prolactin Soluble Receptor Rainbow Trout Recombinant
Cat. No.
BT29884
Source
Escherichia Coli.
Synonyms
PRL-R.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Prolactin Receptor Rainbow Trout Extra Celleular Domain Recombinant ?produced in E.Coli is a non-glycosylated, Polypeptide chain containing 210 amino acids and having a molecular mass of 24034 Dalton.
The Prolactin Receptor is purified by proprietary chromatographic techniques.
The Prolactin Receptor is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Prolactin, a pituitary hormone, plays a crucial role in various physiological processes, including milk production, fluid balance regulation, growth, development, and reproduction. Its action is initiated by binding to a specific cell surface receptor known as the prolactin receptor, which belongs to the class 1 cytokine receptor superfamily. The prolactin receptor's function is mediated, in part, by two signaling pathways: Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). Prolactin (PRL) is involved in diverse functions such as ion transport, osmoregulation, milk and protein synthesis stimulation, and the regulation of multiple reproductive processes. PRL exerts its effects on various cell types by binding to a transmembrane PRL receptor, thereby initiating a signal transduction cascade. Immunoreactive PRL receptor, a member of the cytokine receptor family, exhibits size variations (short and long forms) depending on the tissue source and species, ranging from approximately 40 kDa to 100 kDa. The PRL receptor comprises at least three distinct domains: an extracellular domain containing five cysteine residues and the prolactin binding site, a single transmembrane domain, and a cytoplasmic domain whose length influences ligand binding and modulates cellular function.
Description
Recombinant Prolactin Receptor Extracellular Domain from Rainbow Trout, produced in E. coli, is a non-glycosylated polypeptide chain composed of 210 amino acids, with a molecular weight of 24,034 Daltons. The Prolactin Receptor is purified using proprietary chromatographic methods.
Physical Appearance
White, sterile-filtered lyophilized powder.
Formulation
The protein was lyophilized from a concentrated solution (1 mg/ml) containing 0.0045 mM NaHCO3.
Solubility
To reconstitute the lyophilized PRL-R, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized PRL-R remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, Prolactin Receptor should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to freeze it below -18°C. To enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 97.0% as determined by: (a) Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
Biological activity is assessed by the dose-dependent inhibition of Prolactin-stimulated proliferation of Nb2 cells and by high-affinity binding to ovine Prolactin Receptor (oPLR) and other lactogenic hormones.
Protein Content
Protein quantification was performed using two independent methods: (1) UV spectroscopy at 280 nm, utilizing an absorbance value of 2.48 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value was calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics). (2) Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) analysis, employing a standard solution of PRLr-ECD as a reference standard.
Synonyms
PRL-R.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Arg-His-Thr-Pro.
Product Science Overview
Prolactin Receptor Structure and Function
The prolactin receptor belongs to the superfamily of class 1 cytokine receptors. It consists of at least three separate domains:
- Extracellular Region: Contains five cysteines and the prolactin binding site.
- Transmembrane Domain: A single domain that spans the cell membrane.
- Cytoplasmic Region: The length of this region influences ligand binding and regulates cellular function .
The prolactin receptor mediates its function through two families of signaling molecules: Janus kinases and signal transducers and activators of transcription .
Recombinant Prolactin Soluble Receptor in Rainbow Trout
The Prolactin Soluble Receptor Rainbow Trout Recombinant is a non-glycosylated polypeptide chain produced in Escherichia coli (E. coli). It contains 210 amino acids and has a molecular mass of 24,034 Daltons . The recombinant receptor is purified using proprietary chromatographic techniques .
Physical Appearance and Formulation:
- The protein is provided as a sterile filtered white lyophilized (freeze-dried) powder.
- It is lyophilized from a concentrated solution with 0.0045 mM NaHCO3 .
Solubility and Stability:
- It is recommended to reconstitute the lyophilized prolactin receptor in sterile 18MΩ-cm H2O at a concentration of not less than 100 µg/ml, which can then be further diluted to other aqueous solutions .
- The lyophilized receptor is stable at room temperature for three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for 2-7 days and for future use below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended to prevent freeze-thaw cycles .
Purity and Biological Activity:
- The purity of the recombinant prolactin receptor is greater than 97.0%, as determined by SEC-HPLC and SDS-PAGE analysis .
- Its biological activity is determined by the dose-dependent inhibition of prolactin-stimulated proliferation of Nb2 cells and by high-affinity binding of other lactogenic hormones .
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