PRL R Rabbit

Prolactin Rabbit Soluble Receptor Recombinant
Cat. No.
BT29826
Source
Escherichia Coli.
Synonyms
PRL-R.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Prolactin Receptor Rabbit Extra Celleular Domain Recombinant ?produced in E.Coli is a non-glycosylated, Polypeptide chain containing 207 amino acids and having a molecular mass of 23972 Dalton.
The Prolactin Receptor is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Prolactin, a pituitary hormone, plays a crucial role in various physiological processes, including milk production, fluid balance regulation, growth, development, and reproduction. Its action is initiated by binding to a specific cell surface receptor known as the prolactin receptor, a member of the class 1 cytokine receptor superfamily. The prolactin receptor's function is mediated, in part, by two signaling pathways involving Janus kinases and signal transducers and activators of transcription (STAT) proteins. Prolactin (PRL) is a hormone with diverse functions, including ion transport, osmoregulation, milk and protein synthesis stimulation, and the regulation of reproductive functions. PRL exerts its effects on various cell types by binding to a transmembrane PRL receptor, initiating a signal transduction cascade. The PRL receptor, a member of the cytokine receptor family, exhibits size variations (short and long forms) depending on the tissue source and species, ranging from approximately 40 kDa to 100 kDa. It comprises at least three distinct domains: an extracellular region containing five cysteines and the prolactin binding site, a single transmembrane domain, and a cytoplasmic region, the length of which influences ligand binding and cellular function.
Description
Recombinant Prolactin Receptor Rabbit Extracellular Domain, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 207 amino acids, with a molecular weight of 23972 Daltons. The Prolactin Receptor is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.0045mM NaHCO3.
Solubility
To reconstitute the lyophilized PRL-R, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized PRL-R remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, Prolactin Receptor should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to store it below -18°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% using the following methods:
(a) Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity is determined by assessing the dose-dependent inhibition of Prolactin-stimulated proliferation of Nb2 cells and the high-affinity binding of ovine Prolactin (oPLR) and other lactogenic hormones. References:
1) Bignon et al. (1994) JBC 269; 3318-24
2) Gertler et al. (1996) JBC 271; 24482-91.
Protein Content
Protein content is determined using UV spectroscopy at 280 nm, with an absorbance value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated using the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Synonyms
PRL-R.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Lys-Pro-Phe-Ile.

Product Science Overview

Introduction

The Prolactin Rabbit Soluble Receptor Recombinant is a biochemically engineered protein that plays a crucial role in the study of prolactin and its interactions within biological systems. This receptor is specifically designed for research purposes and is produced using recombinant DNA technology.

Prolactin and Its Receptor

Prolactin is a hormone primarily associated with lactation in mammals. It is produced by the anterior pituitary gland and has various roles, including the regulation of milk production, salt and water balance, growth, development, and reproduction . The prolactin receptor is a protein that binds prolactin, allowing it to exert its effects on target cells.

Production of the Recombinant Receptor

The Prolactin Rabbit Soluble Receptor Recombinant is produced in Escherichia coli (E. coli) as a non-glycosylated polypeptide chain. This recombinant receptor consists of 207 amino acids and has a molecular mass of approximately 23,972 Daltons . The production process involves the use of proprietary chromatographic techniques to purify the receptor .

Structure and Function

The recombinant receptor represents the extracellular domain of the prolactin receptor. This domain is responsible for binding prolactin and mediating its biological effects. By studying the soluble form of the receptor, researchers can gain insights into the mechanisms of prolactin signaling and its role in various physiological processes.

Applications in Research

The Prolactin Rabbit Soluble Receptor Recombinant is widely used in biochemical and pharmacological research. It serves as a valuable tool for:

  • Investigating the binding affinity and specificity of prolactin to its receptor.
  • Studying the signaling pathways activated by prolactin-receptor interactions.
  • Developing and testing potential therapeutic agents targeting prolactin-related disorders.

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