Escherichia Coli.
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim
Greater than 95.0% as determined by SDS-PAGE.
GMCSF Poricne Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids (18-144a.a.) and having a molecular mass of 16.6kDa.
GMCSF is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MAPTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK
Porcine GM-CSF (pGM-CSF) is specifically derived from pigs and has been studied for its unique expression patterns and functional roles in porcine immune cells . The expression of pGM-CSF can be induced by various stimulants such as lipopolysaccharide (LPS), phytohemagglutinin (PHA), and concanavalin A (ConA) . These stimulants trigger the production of pGM-CSF in immune cells like alveolar macrophages and spleen cells .
Recombinant GM-CSF refers to the GM-CSF protein that has been genetically engineered and produced using recombinant DNA technology. This involves cloning the GM-CSF gene and expressing it in a suitable host system, such as bacteria or yeast, to produce the protein in large quantities . The recombinant form of GM-CSF is used in various research and therapeutic applications due to its ability to stimulate the immune system.
A His Tag (Histidine Tag) is a sequence of histidine residues added to proteins to facilitate their purification and detection. The His Tag binds strongly to nickel or cobalt ions, allowing the tagged protein to be easily isolated using affinity chromatography . In the case of recombinant pGM-CSF, the His Tag is added to simplify the purification process and ensure the protein’s purity for research and clinical use .
Recombinant pGM-CSF with a His Tag has been utilized in various studies to understand its immunological functions and potential therapeutic applications. For instance, it has been used to enhance the immunogenicity and antiviral protection of vaccines in piglets . Additionally, the recombinant form of pGM-CSF has shown promise as an adjuvant in swine immunization and viral challenge studies .