GM CSF Human
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.
1. Analysis by RP-HPLC.
2. Analysis by SDS-PAGE.
Description
Product Specs
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the production of white blood cells. It is produced naturally by the body in response to infection or injury, and it can also be produced in the laboratory. GM-CSF is used to treat a variety of conditions, including cancer, AIDS, and bone marrow transplantation. It is also being studied as a treatment for other conditions, such as Crohn's disease and rheumatoid arthritis.
1. Reverse-phase high-performance liquid chromatography (RP-HPLC)
2. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
1. UV spectroscopy at 280 nm using an absorbance value of 0.963 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated using the PC GEN computer analysis program for protein sequences (IntelliGenetics).
2. RP-HPLC analysis using a standard solution of GM-CSF as a reference standard.
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.
N-terminal methionine has been completely removed enzymatically.
Product Science Overview
Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a cytokine that plays a crucial role in the regulation of immune responses. It is produced by various cell types, including activated T cells, NK cells, macrophages, endothelial cells, and fibroblasts . The recombinant form of human GM-CSF (rhu GM-CSF) is used in clinical settings to stimulate the production of white blood cells and enhance immune functions.
GM-CSF is a glycoprotein that functions by binding to its specific receptor on the surface of target cells. This binding activates intracellular signaling pathways that lead to the proliferation, differentiation, and survival of hematopoietic progenitor cells . GM-CSF primarily targets granulocyte and monocyte precursors, as well as subsets of differentiated myeloid cells .
GM-CSF is produced in response to cytokine or immune and inflammatory stimulation. It is secreted by a variety of cells, including T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts . The expression of GM-CSF is tightly regulated and occurs in specific tissues where immune responses are needed.
The primary function of GM-CSF is to stimulate the production and function of granulocytes and macrophages. These cells are essential for the body’s defense against infections and play a critical role in the inflammatory response . GM-CSF also enhances the survival and function of mature myeloid cells, contributing to the overall immune response.
GM-CSF exerts its effects by binding to the GM-CSF receptor, which is composed of an alpha and a beta subunit. This binding triggers a cascade of intracellular signaling events, including the activation of the JAK-STAT pathway, which leads to the transcription of genes involved in cell proliferation, differentiation, and survival .
Recombinant human GM-CSF (rhu GM-CSF), also known as sargramostim, has been approved by the US FDA for use in various clinical settings. It is used to accelerate bone marrow recovery in patients undergoing bone marrow transplantation or chemotherapy . Additionally, rhu GM-CSF has shown promise in enhancing anti-cancer responses when combined with conventional therapies .
