GM CSF Human, His
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
GM-CSF Human Recombinant His is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a cytokine that plays a crucial role in the stimulation of proliferation, differentiation, and survival of various hematopoietic cells, including mature neutrophils, macrophages, and dendritic cells . The recombinant form of GM-CSF, particularly the human recombinant version with a His tag, is widely used in research and therapeutic applications.
GM-CSF is a glycoprotein composed of 127 amino acids. The human recombinant version is often tagged with a polyhistidine (His) tag to facilitate purification and detection. This His tag is a sequence of histidine residues that binds strongly to nickel ions, allowing for easy isolation of the protein using nickel-affinity chromatography.
GM-CSF is produced by various cell types, including fibroblasts, endothelial cells, and T lymphocytes, in response to microbial products or inflammatory cytokines . It is essential for the innate immune response, as it stimulates the proliferation and differentiation of granulocytes and macrophages, which are critical for fighting infections.
The production of recombinant human GM-CSF typically involves the expression of the protein in Escherichia coli (E. coli) cells. However, this process often results in the formation of inclusion bodies, which are aggregates of misfolded proteins . To obtain bioactive GM-CSF, these inclusion bodies must be solubilized, refolded, and purified. A simplified method for the efficient refolding and purification of recombinant human GM-CSF has been developed, which does not require extensive experience in protein refolding or purification .
Recombinant human GM-CSF is used as a biotherapeutic agent for immunocompromised individuals, such as those undergoing chemotherapy . It helps to reduce the duration and severity of neutropenia, a condition characterized by low levels of neutrophils, which can lead to severe infections. By stimulating the production of granulocytes and macrophages, GM-CSF enhances the body’s ability to fight infections and recover from chemotherapy-induced neutropenia.
