GMCSF Porcine
Escherichia Coli.
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.
Greater than 95.0% as determined by SDS-PAGE.
Description
Granulocyte Macrophage-Colony Stimulating Factor Porcine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 128 amino acids and having a molecular mass of 14.5kDa.
GMCSF is purified by proprietary chromatographic techniques.
Product Specs
Granulocyte-macrophage colony-stimulating factor (GMCSF) is a cytokine that plays a crucial role in the production, differentiation, and function of granulocytes and macrophages. The biologically active form of GMCSF exists as a homodimer in the extracellular space. The gene encoding GMCSF is located within a cluster of related genes on chromosome 5q31, a region frequently associated with interstitial deletions observed in 5q- syndrome and acute myelogenous leukemia. This gene cluster also harbors genes encoding other interleukins, namely interleukins 4, 5, and 13.
GM-CSF exerts its effects by stimulating the growth and differentiation of hematopoietic precursor cells across various lineages, encompassing granulocytes, macrophages, eosinophils, and erythrocytes.
Recombinant Porcine Granulocyte-Macrophage Colony-Stimulating Factor, produced in E. coli, is a non-glycosylated monomeric protein comprising 128 amino acids. It exhibits a molecular mass of 14.5 kDa.
The purification of GMCSF is achieved through proprietary chromatographic techniques.
The protein was subjected to lyophilization from a sterile (0.2 µm) filtered solution containing 10 mM sodium phosphate, pH 7.5.
For reconstitution, it is recommended to dissolve the lyophilized GMCSF in sterile 18 MΩ-cm H2O to a concentration of not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized GMCSF, while stable at room temperature for up to 3 weeks, should be stored desiccated at a temperature below -18°C for extended periods. Following reconstitution, GMCSF should be stored at 4°C for a duration of 2-7 days. For long-term storage, it is recommended to store reconstituted GMCSF below -18°C.
Repeated freeze-thaw cycles should be avoided.
The purity of the protein is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
The ED50, determined by TF-1 cell proliferation assay, is 4.52 ng/ml, corresponding to a specific activity of 2.2 x 105 units/mg.
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.
Escherichia Coli.
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Product Science Overview
The recombinant form of porcine GM-CSF is produced using genetic engineering techniques. This involves inserting the gene encoding GM-CSF into a suitable expression system, such as bacteria, yeast, or mammalian cells. The expression system then produces the GM-CSF protein, which can be purified and used for further applications .
GM-CSF is produced by a variety of cell types, including T and B lymphocytes, macrophages, endothelial cells, and fibroblasts . It acts on hematopoietic progenitor cells to stimulate the production of granulocytes (neutrophils, eosinophils, and basophils) and macrophages. These cells are essential components of the immune system, playing key roles in phagocytosis, antigen presentation, and the production of inflammatory mediators.
Recombinant GM-CSF has several applications in both research and clinical settings:
- Research: GM-CSF is used in various in vitro and in vivo studies to understand its role in immune responses, hematopoiesis, and disease mechanisms. It is also used to culture and differentiate immune cells for experimental purposes.
- Therapeutics: GM-CSF is used clinically to enhance the recovery of bone marrow function following chemotherapy or bone marrow transplantation. It is also used to treat certain types of neutropenia (low neutrophil count) and to boost the immune response in patients with infections or immune deficiencies .
In veterinary medicine, recombinant porcine GM-CSF is used to enhance the immune response in pigs, particularly in the context of infectious diseases. It has been shown to improve the viability and developmental competence of porcine embryos during in vitro culture . This application is particularly important for improving reproductive efficiency and health in swine production.
