PLGF3 Human, sf9

Placental Growth Factor-3 Human Recombinant, sf9
Cat. No.
BT28987
Source
Spodoptera frugiperda.
Synonyms
Placental Growth Factor, Placental Growth Factor Vascular Endothelial Growth Factor-Related Protein, PGFL, PLGF, Placental Growth Factor-Like, Placenta Growth Factor, SHGC-10760, D12S1900, PlGF-2, PGF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PLGF3 Human Recombinant produced in Spodoptera frugiperda is a glycosylated homodimer containing 2 chains of 203 amino acids (Leu19-Arg221) and having a molecular mass of 58kDa.
The PLGF-3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
PLGF, a key player in angiogenesis, fuels the growth of endothelial cells by promoting their proliferation and movement. Its binding affinity lies with the receptor known as VEGFR-1/FLT1.
Description
Recombinantly produced in Spodoptera frugiperda, PLGF3 Human takes the form of a glycosylated homodimer. This protein comprises two chains, each consisting of 203 amino acids (Leu19-Arg221), culminating in a molecular weight of 58kDa. The purification of PLGF-3 is achieved through specialized chromatographic techniques.
Physical Appearance
The product appears as a sterile, filtered, and lyophilized powder, white in color.
Formulation
The lyophilization of PLGF-3 protein was carried out using a 0.2µm filtered solution of HCl.
Solubility
For reconstitution, it is advised to dissolve the lyophilized PLGF3 in sterile 4mM HCl at a concentration not lower than 100µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
While lyophilized PLGF3 exhibits stability at room temperature for up to 3 weeks, it is recommended to store it in a desiccated state below -18°C. Upon reconstitution, PLGF-3 can be stored at 4°C for a period of 2-7 days. For extended storage, freezing below -18°C is advised. To ensure optimal long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of the protein surpasses 95.0%, as determined by SDS-PAGE analysis.
Biological Activity
Evaluated through a functional ELISA, the ED50, representing its binding affinity to rHuVEGF R1, is determined to be less than 3ng/ml.
Synonyms
Placental Growth Factor, Placental Growth Factor Vascular Endothelial Growth Factor-Related Protein, PGFL, PLGF, Placental Growth Factor-Like, Placenta Growth Factor, SHGC-10760, D12S1900, PlGF-2, PGF.
Source
Spodoptera frugiperda.

Product Science Overview

Structure and Function

PlGF-3 is primarily expressed in the placental trophoblasts during pregnancy, but it can also be found in other tissues such as the villous trophoblast . It binds to the receptor VEGFR-1 (also known as Flt-1), stimulating endothelial cell growth, proliferation, and migration . This interaction is essential for the proper development of the placenta and the maintenance of a healthy pregnancy.

Recombinant Production

The recombinant form of PlGF-3, produced in sf9 insect cells, is used for research purposes. The sf9 cell line, derived from the fall armyworm, is commonly used in biotechnology for the production of recombinant proteins. This method ensures high yield and proper folding of the protein, making it suitable for various experimental applications .

Applications in Research

PlGF-3 has been extensively studied for its role in pregnancy-related conditions, such as pre-eclampsia. Pre-eclampsia is a serious complication characterized by high blood pressure and proteinuria, which can lead to life-threatening conditions if not properly managed . PlGF-based testing is used to help diagnose and monitor this condition, providing valuable information for the management of at-risk pregnancies .

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