PLGF1 Human
Placental Growth Factor-1 Human Recombinant
Cat. No.
BT28356
Source
Escherichia coli.
Synonyms
PIGF, PGF, PLGF-1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PLGF1 Human Recombinant (21-221 a.a.) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 29kDa.
The PLGF-1 Human Recombinant protein is purified by proprietary chromatographic techniques.
The PLGF-1 Human Recombinant protein is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Placental growth factor (PLGF) is a growth factor involved in angiogenesis and endothelial cell growth, promoting their proliferation and migration. It binds to the vascular endothelial growth factor receptor 1 (VEGFR-1/FLT1).
Description
Recombinant human PLGF1 (amino acids 21-221) produced in E. coli is a disulfide-linked homodimeric, non-glycosylated polypeptide chain consisting of 129 amino acids with a molecular weight of 29 kDa. The recombinant human PLGF1 protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile filtered white lyophilized powder.
Formulation
PLGF1 protein was lyophilized from a 0.2 μm filtered solution containing acetonitrile and trifluoroacetic acid (TFA).
Solubility
It is recommended to reconstitute the lyophilized PLGF1 in sterile 18 MΩ-cm H2O to a concentration of at least 100 μg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized PLGF1 is stable at room temperature for 3 weeks but should be stored desiccated below -18 °C. After reconstitution, PLGF1 should be stored at 4 °C for 2-7 days or below -18 °C for future use. For long-term storage, adding a carrier protein (0.1% human serum albumin or bovine serum albumin) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Greater than 97.0% as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
Binds to immobilized recombinant human VEGFR1 with a half-maximal effective concentration (EC50) of less than 6 ng/ml.
Synonyms
PIGF, PGF, PLGF-1.
Source
Escherichia coli.
Amino Acid Sequence
AVPPQQWALS AGNGSSEVEV VPFQEVWGRS YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RPLREKMKPE RCGDAVPRR.
Product Science Overview
Structure and Function
PlGF-1 is a glycoprotein composed of 149 amino acids. It forms homodimers and heterodimers with VEGF, enhancing its biological activity. The primary function of PlGF-1 is to bind to the VEGF receptor-1 (VEGFR-1), also known as Flt-1, which is expressed on endothelial cells, monocytes, and other cell types. This binding activates several signaling pathways that promote:
- Angiogenesis: Formation of new blood vessels from pre-existing ones.
- Vasculogenesis: Formation of new blood vessels during embryonic development.
- Cell migration and proliferation: Essential for tissue repair and regeneration.
Recombinant Human PlGF-1
Recombinant human PlGF-1 is produced using recombinant DNA technology, which involves inserting the PlGF-1 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of PlGF-1 for research and therapeutic purposes.
Applications in Research and Medicine
Recombinant human PlGF-1 is widely used in scientific research to study its role in various biological processes and diseases. Some key applications include:
- Cancer Research: PlGF-1 is implicated in tumor growth and metastasis. It is used to investigate the mechanisms of tumor angiogenesis and to develop anti-angiogenic therapies.
- Cardiovascular Research: PlGF-1 is involved in the repair of damaged heart tissue and the formation of new blood vessels after a heart attack. It is studied for its potential in treating ischemic heart diseases.
- Wound Healing: PlGF-1 promotes the formation of new blood vessels and tissue regeneration, making it a potential therapeutic agent for chronic wounds and ulcers.
Clinical Implications
The therapeutic potential of PlGF-1 is being explored in various clinical settings. Some potential applications include:
- Treatment of Ischemic Diseases: PlGF-1 could be used to promote blood vessel formation and improve blood flow in patients with ischemic conditions, such as peripheral artery disease and myocardial infarction.
- Cancer Therapy: Targeting PlGF-1 and its signaling pathways may help inhibit tumor growth and metastasis, providing a new avenue for cancer treatment.
- Wound Healing: PlGF-1-based therapies could accelerate the healing of chronic wounds and improve the quality of life for patients with non-healing ulcers.
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