PLGF1 Human, 132 a.a.
Escherichia Coli.
PIGF, PGF, PLGF-1.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Placental Growth Factor-1 Human Recombinant produced in E.Coli is a non-glycosylated, disulfide-linked homodimer consisting of 2x132 amino acid polypeptide chains, having a total molecular mass of approximately 29.7kDa.
PLGF1 is purified by proprietary chromatographic techniques.
Product Specs
PLGF, a key player in angiogenesis, promotes the growth and movement of endothelial cells, essential for blood vessel formation. It exerts its effects by binding to the vegfr-1/flt1 receptor.
Recombinant Human Placental Growth Factor-1, produced in E. coli, is a non-glycosylated, disulfide-linked homodimer. Each protein molecule comprises two polypeptide chains, each consisting of 132 amino acids, resulting in a total molecular weight of approximately 29.7kDa. The purification process involves specialized chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
The product is lyophilized, or freeze-dried, from a concentrated solution (0.2µm filtered) in 10mM Sodium Phosphate buffer with a pH of 7.5.
To reconstitute the lyophilized Placental Growth Factor-1, it is recommended to dissolve it in sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can be further diluted as needed using other aqueous solutions.
Lyophilized PLGF1 remains stable for 3 weeks at room temperature, but for long-term storage, it is recommended to keep it desiccated below -18°C. After reconstitution, Placental Growth Factor-1 should be stored at 4°C for a period of 2-7 days. For extended storage, it should be kept below -18°C. Repeated freezing and thawing of the product should be avoided.
The purity is determined to be greater than 95.0% using the following methods:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
The biological activity is assessed based on the protein's ability to induce chemotaxis, or directed movement, of human monocytes within a concentration range of 1.0-10.0 ng/ml.
PIGF, PGF, PLGF-1.
Escherichia Coli.
MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR.
Product Science Overview
Placental Growth Factor-1 (PlGF-1) is a member of the vascular endothelial growth factor (VEGF) family, which plays a crucial role in angiogenesis and vasculogenesis. This article delves into the background, structure, and significance of PlGF-1, specifically focusing on the human recombinant form consisting of 132 amino acids.
PlGF-1 is involved in various physiological and pathological processes, including:
- Angiogenesis: It promotes the formation of new blood vessels from pre-existing ones.
- Vasculogenesis: It aids in the formation of blood vessels during embryonic development.
- Wound Healing: PlGF-1 accelerates the healing process by enhancing blood supply to the affected area.
- Inflammation: It plays a role in inflammatory responses by recruiting immune cells to the site of inflammation.
PlGF-1 has been extensively studied for its role in pregnancy-related conditions, particularly pre-eclampsia. Pre-eclampsia is a serious complication characterized by high blood pressure and proteinuria during pregnancy . PlGF-based testing is used to diagnose and manage this condition, helping to predict and monitor the risk of developing pre-eclampsia .
The recombinant form of PlGF-1 is produced using E. coli expression systems. This method ensures high purity and activity of the protein, making it suitable for various research and clinical applications . The recombinant protein is typically lyophilized and can be reconstituted for use in experiments .
Recombinant PlGF-1 is used in several research and clinical settings, including:
- Diagnostic Testing: It is used in assays to diagnose and monitor pre-eclampsia.
- Therapeutic Research: Studies are exploring its potential in treating conditions like ischemic heart disease and peripheral artery disease.
- Basic Research: It serves as a tool to study angiogenesis, vasculogenesis, and related cellular processes.
