PLGF Human, HEK
HEK293 cells.
PIGF, PGF, PLGF-1
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
PLGF Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 21-170) containing 160 amino acids and having a molecular mass of 18.3kDa.
PLGF is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
PIGF, PGF, PLGF-1
HEK293 cells.
DGSMAVPPQQ WALSAGNGSS EVEVVPFQEV WGRSYCRALE RLVDVVSEYP SEVEHMFSPS CVSLLRCTGC CGDENLHCVP VETANVTMQL LKIRSGDRPS YVELTFSQHV RCECRPLREK MKPERRRPKG RGKRRREKQR PTDCHLCGDA VPRRHHHHHH
Product Science Overview
The PlGF gene, also known as PGF, is located on chromosome 14 in humans . This gene is primarily expressed in the placenta, specifically in the placental trophoblast, but it is also found in other tissues such as the villous trophoblast . The expression of PlGF is significant during pregnancy, contributing to the development and differentiation of the trophoblast .
There are four known isoforms of PlGF, which arise from alternative splicing of the PGF gene . The main isoforms are PlGF-1 and PlGF-2, which have been extensively studied for their roles in angiogenesis and their potential as biomarkers for pre-eclampsia . PlGF-3 and PlGF-4 are less studied, but they are also present in placental tissue and have unique structural features .
PlGF primarily functions by binding to the VEGF receptor-1 (VEGFR-1), also known as Flt-1 . This interaction stimulates the proliferation and migration of endothelial cells, which are essential for the formation of new blood vessels . PlGF also plays a role in the growth and differentiation of the trophoblast, which is crucial for the proper development of the placenta .
PlGF has been identified as a potential biomarker for various pregnancy-related conditions, including pre-eclampsia and small for gestational age (SGA) fetuses . The levels of PlGF in maternal serum can provide valuable information for the screening and diagnosis of these conditions . Additionally, recombinant PlGF has been studied for its therapeutic potential in enhancing angiogenesis and arteriogenesis in pathological conditions such as myocardial infarction .
Human recombinant PlGF is produced using recombinant DNA technology, which involves inserting the PlGF gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities . This recombinant protein is used in research and clinical applications to study its functions and potential therapeutic uses .
