PEDF PAT13D9AT Antibody

Mouse Anti Human Pigment Epithelium-Derived Factor Clone PAT13D9AT
Cat. No.
BT9659
Source
Synonyms

Pigment epithelium-derived factor,PEDF, Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35.

Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Pigment epithelium-derived factor (PEDF) is a multifaceted protein with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. As a 50 kDa glycoprotein, it is widely produced and secreted by various tissues throughout the body. A key mechanism behind PEDF's anti-angiogenic activity is its ability to induce apoptosis (programmed cell death) in rapidly dividing endothelial cells, which form the lining of blood vessels. Furthermore, PEDF can hinder the action of angiogenic factors like VEGF and FGF-2, which promote blood vessel formation. PEDF exerts its neuroprotective effects by suppressing neuronal apoptosis triggered by stressors such as peroxide, glutamate, and other neurotoxins. The discovery of PEDF-R, a lipase-linked cell membrane receptor that binds specifically and with high affinity to PEDF, is crucial for understanding the intricate workings of this versatile protein. Notably, PEDF-R is the only known signaling receptor utilized by a member of the serpin family, to which PEDF belongs. The diverse range of PEDF's activities makes it a promising candidate for therapeutic intervention in conditions involving blood vessels and neurodegeneration, such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). Moreover, PEDF holds potential for treating various angiogenesis-dependent diseases, including several types of cancer.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1 mg/ml and is dissolved in a buffer consisting of phosphate-buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep the antibody at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored properly at -20°C. At 4°C, the antibody remains stable for 1 month.
Applications
The PEDF antibody has undergone testing in various applications, including ELISA, Western blot analysis, and immunocytochemistry/immunofluorescence (ICC/IF), to confirm its specificity and reactivity. However, optimal working dilutions should be determined empirically for each specific application to achieve the best results.
Synonyms

Pigment epithelium-derived factor,PEDF, Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35.

Purification Method

PEDF antibody was purified by protein-A affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT13D9AT.

Immunogen

Anti-human PEDF mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PEDF protein 20-418 amino acids purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

The Mouse Anti Human Pigment Epithelium-Derived Factor (PEDF) Clone PAT13D9AT is a monoclonal antibody specifically designed to target and bind to the human PEDF protein. PEDF, also known as Serpin F1, is a 50,000 dalton glycoprotein that exhibits neurotrophic, anti-angiogenic, and anti-tumorigenic properties . This protein is secreted by various tissues throughout the body and plays a crucial role in inhibiting angiogenesis by inducing apoptosis in proliferating endothelial cells .

Preparation Methods

The Mouse Anti Human PEDF mAb is produced through the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice that have been immunized with a recombinant human PEDF protein . The recombinant PEDF protein, consisting of amino acids 20-418, is purified from Escherichia coli (E. coli) . The resulting monoclonal antibody is then purified using protein-A affinity chromatography to ensure high specificity and reactivity .

Chemical Reactions Analysis

PEDF’s anti-angiogenic action is primarily mediated through the induction of apoptosis in endothelial cells and the inhibition of angiogenic factors such as VEGF and FGF-2 . Additionally, PEDF has neuro-protective effects by suppressing neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins . The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity has further elucidated the underlying mechanisms of this pluripotent serpin .

Applications

The Mouse Anti Human PEDF Clone PAT13D9AT antibody has been tested and validated for use in various applications, including ELISA, Western blot analysis, and immunocytochemistry/immunofluorescence (ICC/IF) . Its unique range of activities makes it a potential therapeutic agent for treating vasculature-related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR) . Additionally, PEDF has potential applications in treating various angiogenesis-related diseases, including certain cancers .

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