PEDF Antibody
Pigment Epithelium-Derived Factor, Mouse Anti Human
Cat. No.
BT9575
Source
Synonyms
Pigment epithelium-derived factor, PEDF,
Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Pigment epithelium-derived factor (PEDF) is a multifunctional protein with potent neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa secreted glycoprotein found in various tissues throughout the body. One of the critical mechanisms underlying PEDF's anti-angiogenic activity is its ability to induce apoptosis (programmed cell death) specifically in rapidly dividing endothelial cells, which form the lining of blood vessels. This targeted action on endothelial cells effectively inhibits the formation of new blood vessels (angiogenesis). Moreover, PEDF directly counteracts the effects of pro-angiogenic factors like vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2), further suppressing angiogenesis. In addition to its anti-angiogenic role, PEDF exhibits neuroprotective effects by preventing neuronal cell death caused by various insults, including oxidative stress (peroxide), excitotoxicity (glutamate), and other neurotoxins. The discovery of a specific receptor for PEDF on cell surfaces, known as PEDF-R, has significantly advanced our understanding of how this multifaceted protein exerts its diverse effects. PEDF-R, a lipase-linked membrane receptor, binds to PEDF with high affinity, initiating downstream signaling pathways responsible for PEDF's biological activities. Interestingly, PEDF-R is the only known signaling receptor utilized by a member of the serpin family, to which PEDF belongs. The remarkable range of PEDF's biological activities makes it an attractive candidate for therapeutic development, particularly for conditions involving abnormal blood vessel growth or neuronal degeneration.
Formulation
1 mg/ml in PBS, pH 7.4, containing 10% glycerol and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Applications
The PEDF antibody has been validated for ELISA, Western blot analysis, and fluorescence-activated cell sorting (FACS) to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application and experimental setup.
Synonyms
Pigment epithelium-derived factor, PEDF,
Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35.
Type
Mouse Anti Human Monoclonal.
Clone
P18E6AT
Immunogen
Anti-human PEDF mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human PEDF protein 30-504 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Introduction
Pigment Epithelium-Derived Factor (PEDF), also known as Serpin F1 (SERPINF1), is a multifunctional secreted glycoprotein. It belongs to the serine protease inhibitor (serpin) family, although it is a non-inhibitory member. PEDF is widely recognized for its anti-angiogenic, anti-tumorigenic, and neurotrophic properties .
Discovery and Structure
PEDF was first discovered in the late 1980s by Joyce Tombran-Tink and Lincoln Johnson while studying human retinal cell development . It is a 50 kDa protein that is highly conserved across vertebrates. The human PEDF gene is located on chromosome 17 (17p13.3), while the mouse ortholog is found on chromosome 11 .
Biological Functions
PEDF exhibits a broad spectrum of biological activities:
- Anti-Angiogenic Activity: PEDF is a potent inhibitor of angiogenesis. It suppresses blood vessel endothelial cell proliferation and migration, and enhances cell apoptosis .
- Neurotrophic Effects: PEDF promotes neuronal differentiation and survival. It was initially identified for its ability to convert retinoblastoma tumor cells into differentiated non-proliferative neurons .
- Anti-Tumorigenic Properties: PEDF has been shown to possess anti-tumorigenic and anti-metastatic properties. It can act both directly on tumor cells and indirectly by modulating the tumor microenvironment .
Expression and Regulation
Therapeutic Potential
Given its multifunctional properties, PEDF is being researched as a therapeutic candidate for several conditions:
- Cancer Therapy: PEDF’s anti-angiogenic and anti-tumorigenic properties make it a promising candidate for cancer treatment. Novel compounds that upregulate PEDF expression are being explored as potential chemotherapeutic agents .
- Ophthalmic Diseases: PEDF is being investigated for its potential in treating choroidal neovascularization and other retinal diseases .
- Metabolic Disorders: The correlation between PEDF and metabolic syndrome suggests its potential role in treating obesity and insulin resistance .
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