Introduction
Pigment epithelium-derived factor (PEDF) is a multifunctional protein with potent neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa secreted glycoprotein found in various tissues throughout the body. One of the critical mechanisms underlying PEDF's anti-angiogenic activity is its ability to induce apoptosis (programmed cell death) specifically in rapidly dividing endothelial cells, which form the lining of blood vessels. This targeted action on endothelial cells effectively inhibits the formation of new blood vessels (angiogenesis). Moreover, PEDF directly counteracts the effects of pro-angiogenic factors like vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2), further suppressing angiogenesis. In addition to its anti-angiogenic role, PEDF exhibits neuroprotective effects by preventing neuronal cell death caused by various insults, including oxidative stress (peroxide), excitotoxicity (glutamate), and other neurotoxins. The discovery of a specific receptor for PEDF on cell surfaces, known as PEDF-R, has significantly advanced our understanding of how this multifaceted protein exerts its diverse effects. PEDF-R, a lipase-linked membrane receptor, binds to PEDF with high affinity, initiating downstream signaling pathways responsible for PEDF's biological activities. Interestingly, PEDF-R is the only known signaling receptor utilized by a member of the serpin family, to which PEDF belongs. The remarkable range of PEDF's biological activities makes it an attractive candidate for therapeutic development, particularly for conditions involving abnormal blood vessel growth or neuronal degeneration.
Formulation
1 mg/ml in PBS, pH 7.4, containing 10% glycerol and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Applications
The PEDF antibody has been validated for ELISA, Western blot analysis, and fluorescence-activated cell sorting (FACS) to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application and experimental setup.
Synonyms
Pigment epithelium-derived factor, PEDF,
Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human PEDF mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human PEDF protein 30-504 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.