PDGF BB Rat
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The PDGF-BB is purified by proprietary chromatographic techniques.
Product Specs
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Product Science Overview
Platelet-Derived Growth Factor BB (PDGF-BB) is a significant member of the Platelet-Derived Growth Factor family, which plays a crucial role in various cellular processes. PDGF-BB is a homodimer composed of two B chains, and it is known for its potent mitogenic and chemotactic properties. This article delves into the background, structure, function, and applications of PDGF-BB, particularly focusing on the rat recombinant form.
PDGF-BB is a 30 kDa cationic polypeptide consisting of two disulfide-linked B chains . Each chain contains eight cysteine residues, which are involved in intra- and interchain disulfide bonds . The cleavage of these bonds by reduction causes an irreversible loss of biological activity . The A and B chains of PDGF are approximately 40% homologous in sequence and are encoded by distinctly different genes .
PDGF-BB elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes . PDGF-BB is also a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts . It activates smooth muscle cell relocalization and multiplication, which might be associated with gene regulation . Additionally, PDGF-BB increases the synthesis of phospholipids, cholesterol esters, glycogen, and prostaglandins, and modulates low-density lipoprotein (LDL) receptor binding .
Recombinant PDGF-BB from rats is typically expressed in Escherichia coli and is available in a lyophilized powder form suitable for cell culture . The recombinant form is endotoxin tested and has a purity of ≥97% as determined by SDS-PAGE . It is essential to avoid repeated freeze/thaw cycles to maintain its stability and biological activity .
PDGF-BB has been widely used in various research and clinical applications. It serves as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture, and adult spinal cord neurons culture . PDGF-BB is also used in studies related to bone formation, where it acts as a mitogen and chemoattractant for mesenchymal cells and stimulates inflammatory cells such as macrophages to secrete growth factors . Additionally, PDGF-BB has been investigated for its potential to inhibit intervertebral disc degeneration by stimulating tissue cell growth .
