PDGF BB Human

Platelet-Derived Growth Factor BB Human Recombinant
Cat. No.
BT27059
Source
Escherichia Coli.
Synonyms

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Platelet-Derived Growth Factor BB Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.3 kDa. PDGF-BB is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Platelet-derived growth factor BB (PDGF-BB) belongs to the platelet-derived growth factor family, a group of four mitogenic factors known to stimulate the growth of cells with mesenchymal origins. These factors are characterized by a specific motif of eight cysteine residues. PDGF-BB can exist in two forms: as a homodimer (two identical PDGF-BB molecules linked together) or as a heterodimer (one PDGF-BB molecule linked to a platelet-derived growth factor alpha polypeptide, PDGF-AB). Both forms are connected by disulfide bonds. Mutations in the gene responsible for producing PDGF-BB have been linked to meningioma, a type of tumor that affects the brain and spinal cord. Additionally, reciprocal translocations (a specific type of chromosomal rearrangement) involving chromosomes 22 and 7, particularly at the locations of the PDGF-BB gene and the COL1A1 gene (responsible for collagen production), are associated with dermatofibrosarcoma protuberans (DFSP). DFSP is a rare, slow-growing tumor of the skin and underlying tissues, often resulting from the uncontrolled expression of growth factors like PDGF-BB. Interestingly, two different splice variants of the PDGF-BB gene have been identified.
Description
Recombinant Human Platelet-Derived Growth Factor BB is a laboratory-produced protein that mimics the structure and function of naturally occurring PDGF-BB. It exists as a homodimer, meaning it consists of two identical polypeptide chains, each composed of 109 amino acids, totaling 218 amino acids. This non-glycosylated protein has a molecular weight of 24.3 kDa and undergoes a purification process using specialized chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The product is freeze-dried (lyophilized) from a concentrated solution (0.2µm filtered) prepared in phosphate-buffered saline (PBS) at a pH of 7.4.
Solubility

To reconstitute (dissolve) the lyophilized Platelet-derived Growth Factor-BB, it is recommended to use sterile 18 MΩ-cm H2O (high purity water) at a concentration of at least 100 µg/ml. This solution can be further diluted using other aqueous solutions if needed.

Stability
Lyophilized Platelet-derived Growth Factor BB, while remaining stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C for long-term preservation. Once reconstituted, PDGF BB can be stored at 4°C for 2-7 days. For extended storage, freezing below -18°C is recommended. However, repeated freeze-thaw cycles should be avoided.
Purity
The purity of this product is greater than 95%, determined by two analytical methods:
(a) High-performance liquid chromatography (RP-HPLC)
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
Biological Activity
The biological activity of this product is assessed by its ability to stimulate the growth of Balb/c 3T3 cells in a dose-dependent manner. This is measured as the effective concentration (ED50) at which the product induces a 50% increase in cell proliferation, typically falling within the range of 1.0-3.0 ng/ml.
Synonyms

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide.

Source
Escherichia Coli.
Amino Acid Sequence
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.

Product Science Overview

Introduction

Platelet-Derived Growth Factor BB (PDGF-BB) is a member of the PDGF family, which plays a crucial role in various biological processes, including development, cell proliferation, cell survival, and angiogenesis . PDGF-BB is a cystine-linked homodimer, meaning it consists of two identical subunits linked by disulfide bonds .

Expression Patterns and Tissue Distribution

PDGF-BB is expressed by several cell types, including vascular endothelial cells, megakaryocytes, and Leydig cells . Its expression is particularly significant in tissues involved in wound healing and tissue repair, where it acts as a potent mitogen for cells of mesenchymal origin .

Biological Functions

PDGF-BB has several key biological functions:

  • Cell Proliferation: It stimulates the proliferation of various cell types, including fibroblasts and smooth muscle cells .
  • Cell Survival: PDGF-BB promotes cell survival by activating anti-apoptotic pathways .
  • Angiogenesis: It plays a vital role in the formation of new blood vessels, which is essential for tissue repair and regeneration .
Mode of Action

PDGF-BB exerts its effects by binding to PDGF receptors on the surface of target cells. This binding activates intracellular signaling pathways that lead to various cellular responses, including proliferation, migration, and survival . The primary receptors for PDGF-BB are PDGFR-α and PDGFR-β, which are tyrosine kinase receptors .

Regulatory Mechanisms

The activity of PDGF-BB is tightly regulated at multiple levels:

  • Gene Expression: The expression of PDGF-BB is controlled by various transcription factors that respond to cellular signals and environmental cues .
  • Protein Processing: PDGF-BB is synthesized as a precursor protein that undergoes proteolytic cleavage to become active .
  • Receptor Interaction: The availability of PDGF receptors on the cell surface and their affinity for PDGF-BB are critical determinants of its biological activity .
Clinical Applications

Recombinant human PDGF-BB (rhPDGF-BB) has been developed for therapeutic use, particularly in the field of regenerative medicine. It is used in combination with osteoconductive matrices, such as beta-tricalcium phosphate (β-TCP), to promote bone healing and tissue regeneration . Clinical trials have demonstrated that rhPDGF-BB is a safe and effective alternative to autografts for bone grafting procedures .

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