PDGF BB Human, Yeast
Description
PDGF-BB is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Platelet-Derived Growth Factor-BB (PDGF-BB) is a significant member of the PDGF family, which plays a crucial role in various cellular processes, including proliferation, differentiation, and migration. PDGF-BB is a homodimer composed of two B chains, and it is known for its potent mitogenic effects on mesenchymal cells, such as fibroblasts and smooth muscle cells.
Recombinant human PDGF-BB (rhPDGF-BB) is produced using yeast expression systems, particularly Saccharomyces cerevisiae and Pichia pastoris. These systems are favored due to their ability to perform post-translational modifications and their ease of genetic manipulation. The production process involves inserting the gene encoding the PDGF-B chain into the yeast genome, allowing the yeast to express and secrete the protein.
The purification of rhPDGF-BB involves several chromatographic techniques to achieve high purity levels. For instance, a study optimized the purification process using Pichia pastoris, resulting in rhPDGF-BB with a purity of over 98% . The purified protein is characterized by its molecular weight, glycosylation patterns, and biological activity. Glycosylation, particularly O-linked glycosylation, can occur during the secretion process in yeast, affecting the protein’s structure and function .
PDGF-BB is known for its ability to stimulate cell proliferation and migration, making it valuable in regenerative medicine. It has been approved for medical use in various applications, including the treatment of neuropathic ulcers, severe skin burns, and periodontal surgery . The protein’s mitogenic activity is measured using assays such as the WST-1 proliferative assay, which assesses its effect on cell cultures .
One of the notable medical applications of rhPDGF-BB is in the drug becaplermin, which is used for treating diabetic foot ulcers . Additionally, PDGF-BB is utilized in combination with osteoconductive matrices for periodontal surgery and tissue engineering. Its role in promoting wound healing and tissue regeneration makes it a critical component in various therapeutic formulations.
