PDGF A Human
Platelet-Derived Growth Factor A Human Recombinant
Cat. No.
BT26373
Source
Escherichia Coli.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-A, PDGF-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Platelet-Derived Growth Factor A Human Recombinant short chain produced in E.Coli is a non-glycosylated, polypeptide chain containing 110 amino acids fragment (87-196) and having a total Mw of 17.02kDa, with an amino-terminal hexahistidine tag.
PDGF-A is purified by proprietary chromatographic techniques.
PDGF-A is purified by proprietary chromatographic techniques.
Product Specs
Introduction
The term 'PDGF' refers to a family of disulphide bond-linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. The protein also contributes to wound healing and neural regeneration. There are four members of the PDGF family – PDGF A, PDGF B, PDGF C and PDGF D. Two distinct types of PDGF-A exist – a short form that is soluble and a long form that is retained by the extracellular matrix.
Description
Platelet-Derived Growth Factor A Human Recombinant short chain produced in E.Coli is a non-glycosylated, polypeptide chain containing 110 amino acids fragment (87-196) and having a total Mw of 17.02kDa, with an amino-terminal hexahistidine tag. PDGF-A is purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered clear solution.
Formulation
Platelet-derived Growth Factor A is supplied in 25mM Na-Acetate, pH-4.8, 1mM EDTA and 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-A, PDGF-1.
Source
Escherichia Coli.
Product Science Overview
Biological Properties and Functions
PDGF-A is integrally involved in several critical biological processes, including:
- Embryonic Development: PDGF-A is essential for the development of various tissues and organs during embryogenesis .
- Angiogenesis: It promotes the formation of new blood vessels, which is vital for tissue growth and repair .
- Organogenesis: PDGF-A plays a role in the formation and development of organs .
- Fibroblast Proliferation and Migration: It induces the proliferation and migration of fibroblasts, which are crucial for wound healing and tissue repair .
Mechanism of Action
PDGF-A exerts its effects by binding to the PDGF receptor alpha (PDGFR-α), a receptor tyrosine kinase. This binding activates several intracellular signaling pathways, including:
Production and Purification
Clinical Applications
Recombinant PDGF-A has shown promise in various clinical applications, particularly in regenerative medicine. It is used to promote the healing of periodontal and orthopedic bone defects, as well as in dermal wound healing . The use of recombinant PDGF-A in combination with bone grafts has demonstrated significant clinical benefits in tissue regeneration .
Regulatory Mechanisms
The expression and activity of PDGF-A are tightly regulated at multiple levels, including:
- Transcriptional Regulation: Various transcription factors and signaling molecules regulate the expression of PDGF-A genes .
- Post-translational Modifications: PDGF-A undergoes proteolytic cleavage to form the active protein, and its activity can be modulated by glycosylation and other modifications .
- Extracellular Matrix Binding: PDGF-A can bind to components of the extracellular matrix, which influences its availability and activity in the tissue microenvironment .
Dysregulation and Disease
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