PDGF BB Mouse

Platelet-Derived Growth Factor BB Mouse Recombinant
Cat. No.
BT27148
Source
Escherichia Coli.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Platelet-Derived Growth Factor BB Mouse Recombinant produced in E.coli, is a homodimeric, non-glycosylated, polypeptide chain containing 2x110 (total of 2 chains 220aa) amino acids and having a total molecular weight of 24.7 kDa.
PDGF-BB is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Platelet-derived growth factor BB (PDGF-BB) belongs to the platelet-derived growth factor family, known for stimulating the growth of mesenchymal cells. These factors are characterized by a conserved eight-cysteine motif. PDGF-BB exists as a homodimer or forms a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), linked by disulfide bonds. Genetic alterations in PDGF-BB are linked to meningioma. Additionally, chromosomal translocations involving the PDGF-BB gene on chromosome 22 and the COL1A1 gene on chromosome 7 are associated with a skin tumor, dermatofibrosarcoma protuberans, characterized by uncontrolled growth factor expression. Two splice variants of the PDGF-BB gene have been identified.
Description
Recombinant Mouse Platelet-Derived Growth Factor BB, produced in E.coli, is a non-glycosylated homodimeric polypeptide comprising two chains of 110 amino acids each (total 220 amino acids). It has a molecular weight of 24.7 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile-filtered.
Formulation
The protein was lyophilized from a solution of 10mM Sodium Citrate, pH 3.0.
Solubility
To reconstitute the lyophilized Platelet-derived Growth Factor-BB, it is recommended to dissolve it in sterile 100mM acetic acid containing 0.1% BSA to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Platelet-derived Growth Factor BB, though stable at room temperature for 3 weeks, should be stored in a dry environment below -18°C. After reconstitution, PDGF-BB should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% using the following methods:
(a) Reverse-phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity is assessed by measuring the dose-dependent stimulation of human umbilical vein endothelial cell (HUVEC) proliferation. This is determined within a concentration range of less than 1.6 ng/ml.
Protein Content
Protein quantification is performed using two independent methods:
1. UV spectroscopy at 280 nm. An absorbance value of 0.48 is used as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics).
2. RP-HPLC analysis, employing a standard solution of PDGF-BB as a reference standard.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.
Source
Escherichia Coli.
Amino Acid Sequence

MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT

Product Science Overview

Introduction

Platelet-Derived Growth Factor BB (PDGF-BB) is a member of the PDGF family, which plays a crucial role in various biological processes, including embryonic development, cell proliferation, cell migration, and angiogenesis . PDGF-BB is one of the isoforms of the PDGF family, which also includes PDGF-AA, PDGF-CC, PDGF-DD, and the heterodimer PDGF-AB .

Structure and Function

PDGF-BB is a homodimer composed of two B chains, each with a molecular weight of approximately 12.5 kDa, resulting in a total molecular weight of 25.0 kDa . The protein is produced in Escherichia coli (E. coli) and purified via sequential chromatography . PDGF-BB signals through receptor tyrosine kinases, specifically PDGFRα and PDGFRβ .

Biological Activity

PDGF-BB is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells . It has been shown to induce mesenchymal stem cell migration and angiogenesis, which are essential for proper osteogenesis during bone modeling and remodeling . Additionally, PDGF-BB is involved in the pathogenesis of various diseases, including atherosclerosis, glomerulonephritis, and cancer .

Applications

Recombinant mouse PDGF-BB is widely used in research to study its effects on cell proliferation, migration, and angiogenesis. It is also utilized in tissue engineering and regenerative medicine to promote tissue repair and regeneration .

Storage and Handling

The lyophilized protein should be stored at 2–8°C or −20°C for long-term storage, preferably desiccated . Upon reconstitution, it should be apportioned into working aliquots and stored at ≤ −20°C to avoid repeated freeze-thaw cycles .

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