PDGF AB Human
Description
PDGF-AB is purified by proprietary chromatographic techniques.
Product Specs
Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT.
Product Science Overview
PDGF-AB is synthesized by megakaryocytes and stored in the alpha granules of platelets. Upon platelet activation, typically triggered by thrombin or other inflammatory signals such as IL-1, IL-6, and TNF-α, PDGF-AB is released into the surrounding tissue . Besides platelets, PDGF-AB is also produced by macrophages, endothelial cells, and fibroblasts, and is present in the nervous system and muscles .
PDGF-AB exerts its biological effects by binding to PDGF receptors alpha and beta (PDGFR-α and PDGFR-β). This binding activates a cascade of intracellular signaling pathways that promote cell proliferation, migration, and survival. PDGF-AB acts as a potent mitogen for mesenchymal-derived cells, including fibroblasts, smooth muscle cells, and cells of the connective tissue .
PDGF-AB is involved in several critical physiological processes:
- Wound Healing: It promotes the proliferation and migration of fibroblasts and smooth muscle cells to the site of injury, facilitating tissue repair and regeneration .
- Development of the Nervous System: PDGF-AB plays a role in the development and maintenance of the nervous system .
- Osteogenic Differentiation: It is involved in the differentiation of mesenchymal stem cells into osteoblasts, contributing to bone formation .
Due to its significant role in cell proliferation and tissue regeneration, PDGF-AB is extensively studied for its potential therapeutic applications. It is used in various research and clinical settings, including:
- Cell Culture and Differentiation Studies: PDGF-AB is utilized to study the differentiation of mesenchymal stem cells and other cell types .
- Wound Healing Assays: It is employed in assays to evaluate the wound healing potential of various treatments .
- Tissue Engineering: PDGF-AB is explored as a therapeutic target for tissue regeneration and repair .
Recombinant PDGF-AB is produced using advanced biotechnological methods, typically involving the expression of the protein in Escherichia coli (E. coli). The recombinant protein is then purified using proprietary chromatographic techniques to ensure high purity and biological activity . Recombinant PDGF-AB is available in various formulations and is used for research purposes to study its biological functions and potential therapeutic applications .
