PDGF AA Mouse
Platelet-derived Growth Factor AA Mouse Recombinant
Cat. No.
BT26605
Source
Escherichia Coli.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
PDGF-AA Mouse Recombinant is a disulfide linked homodimeric, non-glycosylated, polypeptide chain containing 2 x 126 amino acids and having a total molecular mass of 28.9 kDa.
PDGF-AA is purified by proprietary chromatographic techniques.
PDGF-AA is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Platelet-derived growth factors (PDGFs), including PDGF-AA, PDGF-BB, and PDGF-AB, are potent stimulators of cell division for various cell types, such as smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. Stored within platelet alpha-granules, PDGF is released upon platelet activation. It plays a role in several biological processes, including tissue growth, cell migration, development of neurons in embryos, and the formation of respiratory tubules. Two different receptors, PDGFR-alpha and PDGFR-beta, mediate PDGF signaling. While PDGFR-alpha exhibits high affinity for all three PDGF forms, PDGFR-beta interacts specifically with PDGF-BB and PDGF-AB.
Description
Recombinant Mouse PDGF-AA is a protein consisting of two identical polypeptide chains, each composed of 126 amino acids, linked together by a disulfide bond. This non-glycosylated protein has a molecular weight of 28.9 kDa. The purification of PDGF-AA is achieved using proprietary chromatographic methods.
Physical Appearance
White powder, sterile-filtered and lyophilized (freeze-dried).
Formulation
The protein was lyophilized without any additional ingredients.
Solubility
To reconstitute the lyophilized PDGF-AA, it is recommended to dissolve it in sterile water with a resistance of 18MΩ-cm at a concentration of 0.1-0.5mg per ml. This solution can then be further diluted as needed using other aqueous solutions.
Stability
Lyophilized PDGF-AA remains stable for 3 weeks when stored at room temperature. However, for long-term storage, it is recommended to store it in a dry environment below -18°C. Once reconstituted, PDGF-AA should be kept at 4°C for 2-7 days. For longer storage periods, store it below -18°C. To enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advisable. Avoid repeated freezing and thawing cycles.
Purity
The purity of the product is greater than 97%, as determined by two methods: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of the product is determined by its ability to stimulate the proliferation of Balb/c 3T3 cells in a dose-dependent manner. The expected effective concentration for achieving 50% of the maximum response (ED50) is in the range of 8-10 ng/ml, corresponding to a specific activity of 100,000-125,000 IU/mg.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Source
Escherichia Coli.
Amino Acid Sequence
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT.
Product Science Overview
Structure and Function
PDGF-AA is a homodimer composed of two A chains. It is a potent mitogen, meaning it stimulates cell division, particularly for cells of mesenchymal origin such as fibroblasts, glial cells, and vascular smooth muscle cells . The PDGF-AA protein is synthesized, stored, and released by platelets upon activation. It can also be expressed by epithelial cells, smooth muscle cells, and neuronal progenitors .
Receptor Interaction
PDGF-AA exerts its biological effects by binding to its specific receptor, PDGFR-alpha (PDGFRα). This interaction triggers a cascade of intracellular signaling pathways, including the MEK/ERK, EGFR, Src, and PI3K/AKT pathways . These pathways are crucial for various cellular processes such as proliferation, differentiation, and migration.
Biological Roles
PDGF-AA plays a significant role in several physiological and pathological processes:
- Embryonic Development: It directs cell migration during embryonic development and postnatal migration responses, including wound healing .
- Oligodendrocyte Progenitor Cells: PDGF-AA is known to promote the proliferation of oligodendrocyte progenitor cells (OPCs) and their differentiation into oligodendrocytes by activating the ERK pathway .
- Cancer: PDGF-AA has been implicated in the pathogenesis of various cancers, including non-small cell lung carcinomas, where it acts as an autocrine regulator of vascular endothelial growth factor (VEGF) expression .
- Other Diseases: It is also involved in the pathogenesis of atherosclerosis, glomerulonephritis, and the contraction of vascular smooth muscle cells .
Recombinant PDGF-AA
Recombinant PDGF-AA is produced using various expression systems, including E. coli. The recombinant protein typically consists of amino acids Ser87-Thr211 and has a molecular mass of approximately 14 kDa . It is used extensively in research to study its effects on cell proliferation, differentiation, and migration.
Applications
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