PDGF AA Rat

Platelet-Derived Growth Factor AA Rat Recombinant
Cat. No.
BT26685
Source
Escherichia Coli.
Synonyms
Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Pdgfa, Rpa1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Platelet-derived Growth Factor AA Human Recombinant is a disulfide-linked homodimer Consists of two A chains containing 111 amino acids each and having a total molecular mass of 25.3KDa. PDGF-AA is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Platelet-derived growth factors (PDGFs) are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. They are stored in platelet alpha-granules and released upon platelet activation. PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. All three PDGF isoforms bind with high affinity to PDGFR-alpha. In contrast, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
Description
Platelet-derived Growth Factor AA Rat Recombinant is a disulfide-linked homodimer Consists of two A chains containing 110 amino acids each and having a total molecular mass of 24.2 kDa. PDGF-AA is purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
PDGF-AA was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.0.
Solubility
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in sterile 4mM HCl to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Stability
Lyophilized Platelet-derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity
The ED50, as determined by a cell proliferation assay using murine Balb/c 3T3 cells, is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Synonyms
Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Pdgfa, Rpa1.
Source
Escherichia Coli.
Amino Acid Sequence
MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R.

Product Science Overview

Introduction

Platelet-Derived Growth Factor (PDGF) is a significant growth factor involved in various cellular processes, including proliferation, differentiation, and migration. PDGF-AA is one of the isoforms of PDGF, consisting of two identical A subunits. This article delves into the background of PDGF-AA, particularly focusing on its recombinant form derived from rats.

Structure and Function

PDGF-AA is a dimeric protein composed of two A subunits linked by disulfide bonds. It primarily signals through the PDGFRα receptor, inducing mesenchymal cell growth, morphogenesis, and migration . PDGF-AA plays a crucial role in the development of several cell types and tissues, including fibroblasts, glial cells, and vascular smooth muscle cells .

Recombinant PDGF-AA

Recombinant PDGF-AA is produced using recombinant DNA technology, where the gene encoding the PDGF-AA protein is inserted into a host organism, typically E. coli, to produce the protein in large quantities. This recombinant form is optimized for use in various research applications, including cell culture, differentiation studies, and functional assays .

Applications

Recombinant PDGF-AA is widely used in scientific research due to its potent mitogenic effects on cells of mesenchymal origin. Some of its key applications include:

  • Cell Culture: Used to stimulate the growth and proliferation of mesenchymal cells in vitro.
  • Differentiation Studies: Helps in studying the differentiation pathways of various cell types.
  • Functional Assays: Employed in assays to investigate the biological activity and signaling pathways of PDGF-AA .
Biological Activity

The biological activity of recombinant PDGF-AA is typically measured using cell proliferation assays. For instance, it has been shown to stimulate the proliferation of NR6R-3T3 mouse fibroblast cells in a dose-dependent manner . The effective dose (ED50) for this effect ranges from 50 to 200 ng/mL .

Clinical Implications

PDGF-AA has been implicated in various pathological conditions, including atherosclerosis, glomerulonephritis, and cancer. Dysregulation of PDGF signaling can lead to abnormal cell growth and tissue development . Recombinant PDGF-AA is also used in therapeutic applications, such as promoting wound healing and tissue regeneration .

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