p53 Human

The tumor suppressor protein p53 plays a critical role in responding to cellular stress. It achieves this by regulating specific target genes, which in turn can trigger various cellular responses, including cell cycle arrest, programmed cell death (apoptosis), cellular senescence, DNA repair, and metabolic changes. Found in a wide range of tissues, p53 is crucial for regulating cell growth, replication, and apoptosis. This protein possesses distinct domains for DNA binding, transcription activation, and oligomerization. Notably, p53 interacts with other proteins such as mdm2, SV40 T antigen, and the human papillomavirus E6 protein. By sensing DNA damage, p53 potentially facilitates DNA repair mechanisms. Encoded by the TP53 gene in humans, p53 functions as a transcription factor. Alterations in the TP53 gene are implicated not only in somatic mutations within various human cancers but also in germline mutations found in families predisposed to cancer, such as those with Li-Fraumeni syndrome. Mutations affecting p53 are prevalent in diverse human cancers, including breast, ovarian, bladder, colon, lung, and melanoma. These mutations often hinder the protein's ability to bind to its consensus DNA binding site, thereby impairing its tumor suppressor function. While p53 expression remains low in healthy cells, it is elevated in various transformed cell lines, suggesting a potential contribution to cellular transformation and malignancy. Adding complexity to its function, multiple p53 variants exist, encoding distinct isoforms that can modulate p53 transcriptional activity. Due to its crucial role in cell cycle regulation and its contribution to preventing cancer development, p53 is recognized as a vital tumor suppressor. Its ability to preserve genomic stability by preventing mutations has earned it the titles of "the guardian of the genome," "the guardian angel gene," and the "master watchman." The name p53 originates from its apparent molecular mass: it migrates as a 53kDa protein on SDS-PAGE. However, based on its amino acid composition, p53's actual mass is 43.7kDa. This discrepancy arises from the protein's high proline content. Proline residues slow down its migration during SDS-PAGE, making it appear larger than its true size.

This product consists of full-length recombinant human p53 protein. Produced in E.Coli, it is non-glycosylated and has a molecular weight of 81kDa. This p53 protein is fused to a GST tag and purified using proprietary chromatographic techniques.

This product appears as a clear solution that has been sterilized by filtration.

This product contains purified human p53 in a buffer of 50mM Tris-HCl at a pH of 7.5, along with 10mM L-glutathione (reduced).

To ensure long-term storage, keep this product at a temperature of -20°C. It is important to avoid repeated freeze-thaw cycles.

Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53, P53, LFS1, TRP53, FLJ92943.

Escherichia Coli.