p53 scFv antibody
Description
This tumour suppressor gene is mutated in about half of all human cancers.
Recombinant Anti p53 produced in E.Coli is a non-glycosylated, polypeptide chain containing a hexahistidine tag and having a molecular weight of 37 kDa.
The rAp53 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The p53 scFv recombinant antibody is a single-chain variable fragment (scFv) that specifically targets the p53 protein. The p53 protein, also known as tumor protein p53, is a crucial tumor suppressor involved in preventing cancer formation. It plays a significant role in regulating the cell cycle and inducing apoptosis in response to DNA damage.
The p53 scFv recombinant antibody is engineered to consist of the variable regions of the heavy (VH) and light (VL) chains of an antibody, connected by a flexible peptide linker. This design allows the scFv to retain the antigen-binding specificity of a full-length antibody while being smaller and more stable.
The p53 protein is often referred to as the “guardian of the genome” due to its role in maintaining genomic stability. It is activated in response to various cellular stresses, such as DNA damage, hypoxia, and oncogene activation. Once activated, p53 can induce cell cycle arrest, DNA repair, or apoptosis, depending on the context and severity of the damage.
Recombinant antibodies, including scFvs, are produced using genetic engineering techniques. The genes encoding the VH and VL regions are cloned into an expression vector, which is then introduced into a host cell, such as Escherichia coli (E. coli) or mammalian cells. The host cells express the scFv, which can be purified using affinity chromatography.
One of the significant advantages of scFvs is their ability to be produced in large quantities without the need for animal immunization. This makes them a valuable tool for research, diagnostics, and therapeutic applications. The production process can be optimized to achieve high yields and purity, ensuring the recombinant antibody’s effectiveness and reliability.
The p53 scFv recombinant antibody has several applications in research and medicine:
- Cancer Research: Given the pivotal role of p53 in cancer biology, the p53 scFv is widely used to study the mechanisms of p53 regulation and function. It can help identify potential therapeutic targets and develop new cancer treatments.
- Diagnostics: The p53 scFv can be used in diagnostic assays to detect p53 mutations or alterations in cancer patients. This can aid in early diagnosis and personalized treatment strategies.
- Therapeutics: Recombinant antibodies, including scFvs, are being explored as potential therapeutic agents. The p53 scFv can be engineered to deliver cytotoxic agents specifically to cancer cells expressing mutant p53, minimizing damage to healthy cells.
The p53 scFv recombinant antibody offers several advantages over traditional monoclonal antibodies:
- Smaller Size: The smaller size of scFvs allows for better tissue penetration and faster clearance from the body.
- Ease of Production: scFvs can be produced in large quantities using bacterial or mammalian expression systems, reducing the reliance on animal immunization.
- Engineering Flexibility: The genetic engineering of scFvs allows for the introduction of modifications to enhance their stability, affinity, and specificity.
However, there are also challenges associated with scFvs:
- Stability: scFvs can be less stable than full-length antibodies, requiring optimization to improve their stability and shelf-life.
- Affinity: The binding affinity of scFvs may be lower than that of full-length antibodies, necessitating engineering efforts to enhance their binding properties.
