p53 Antibody
Description
Product Specs
Product Science Overview
The p53 protein, often referred to as the “guardian of the genome,” is a crucial tumor suppressor involved in preventing cancer formation. It plays a significant role in regulating the cell cycle and inducing apoptosis in response to DNA damage. The p53 mouse antibody is a monoclonal antibody used extensively in research to study the p53 protein’s function and regulation.
The p53 protein is a sequence-specific transcription factor that is activated by various cellular stress signals, including DNA damage, oxidative stress, and oncogene activation. It consists of several functional domains:
- N-terminal transactivation domain: Involved in the activation of transcription.
- Central DNA-binding domain: Binds to specific DNA sequences to regulate target gene expression.
- Oligomerization domain: Facilitates the formation of p53 tetramers, which are essential for its activity.
- C-terminal regulatory domain: Modulates the protein’s stability and activity.
Upon activation, p53 can induce cell cycle arrest, allowing time for DNA repair, or trigger apoptosis if the damage is irreparable. This dual role makes p53 a critical player in maintaining genomic integrity and preventing tumor development .
The p53 mouse antibody is a monoclonal antibody developed to detect the p53 protein in various applications, including Western blotting, immunoprecipitation, immunofluorescence, flow cytometry, and chromatin immunoprecipitation. These antibodies are typically produced by immunizing mice with a synthetic peptide corresponding to a specific region of the p53 protein .
The p53 mouse antibody is widely used in research to:
- Detect p53 expression: In various cell types and tissues, helping to understand its role in different biological contexts.
- Study p53 regulation: By examining its interaction with other proteins, such as MDM2, which targets p53 for ubiquitination and degradation .
- Investigate p53 mutations: Common in many cancers, providing insights into how these mutations affect p53 function and contribute to tumorigenesis .
- Explore p53-mediated pathways: Including cell cycle arrest, DNA repair, and apoptosis, to develop potential therapeutic strategies for cancer treatment .
