NUDT5 Human

NUDT5 is a human recombinant protein produced in Escherichia coli. It is a single, non-glycosylated polypeptide chain consisting of 239 amino acids, with a molecular mass of approximately 26.5 kDa . The recombinant form of NUDT5 is often fused to a 20 amino acid His-Tag at the N-terminus to facilitate purification through chromatographic techniques .

NUDT5 primarily functions as an ADP-sugar pyrophosphatase. It hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium ions. Additionally, it can hydrolyze other nucleotide sugars, such as ADP-glucose and diadenosine diphosphate, albeit with decreasing activity . The central Nudix motif within NUDT5 is essential for its enzymatic activity, enabling it to eliminate toxic nucleotide derivatives from the cell while maintaining the levels of signaling nucleotides .

NUDT5 is broadly expressed across various tissues but is most abundant in the liver, where it exists as a homodimer . This widespread expression underscores its importance in cellular metabolism and detoxification processes.

The recombinant NUDT5 protein is typically formulated as a sterile, filtered, colorless solution containing 20 mM Tris-HCl (pH 8), 1 mM DTT, 0.1 M NaCl, and 10% glycerol . For short-term storage, it is recommended to keep the protein at 4°C if it will be used within 2-4 weeks. For long-term storage, the protein should be frozen at -20°C, with the addition of a carrier protein (such as 0.1% HSA or BSA) to prevent degradation .

NUDT5 is primarily used in laboratory research to study its role in nucleotide metabolism and its potential implications in various diseases. It is important to note that products containing NUDT5 are intended for research use only and are not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .