NUDT21 Human
Description
Product Specs
Product Science Overview
Nudix Type Motif 21, also known as NUDT21, is a member of the Nudix hydrolase family. Nudix hydrolases are a superfamily of enzymes that catalyze the hydrolysis of nucleoside diphosphates linked to other moieties, commonly referred to as “X” (hence the name Nudix: Nucleoside Diphosphate linked to X). These enzymes play a crucial role in cellular metabolism by eliminating potentially harmful nucleotide derivatives .
NUDT21 is a human recombinant protein that is produced in Escherichia coli. It is a single, non-glycosylated polypeptide chain consisting of 247 amino acids and has a molecular mass of approximately 28.3 kDa . The protein includes a 20 amino acid His tag at the N-terminus, which facilitates its purification through chromatographic techniques .
The Nudix motif, which is characteristic of this family, is a conserved sequence that includes catalytic and metal-binding amino acids. The typical sequence for the Nudix motif is GXXXXXEXXXXXXXREUXEEXGU, where U represents isoleucine, leucine, or valine, and X can be any amino acid . This motif forms a short helix that usually contains the catalytic amino acids necessary for the enzyme’s activity.
NUDT21 is involved in the cleavage and polyadenylation specificity factor (CPSF) complex, specifically as the 25 kDa subunit. It plays a vital role in pre-mRNA 3’ end processing, which is essential for the maturation of messenger RNA (mRNA) molecules . The protein localizes to paraspeckles and forms a heterodimer with CPSF6 or CPSF7, comprising the mammalian cleavage factor I (CFIm) complex . This complex is crucial for the regulation of alternative polyadenylation, a process that influences the stability, localization, and translation efficiency of mRNA.
The recombinant form of NUDT21 is used extensively in research to study its function and role in mRNA processing. It is also utilized in various biochemical assays to understand the mechanisms of Nudix hydrolases and their involvement in cellular metabolism. The availability of human recombinant NUDT21 allows for detailed structural and functional analyses, contributing to our understanding of its role in gene expression regulation.
