NUDT3 Human

Nudix Type Motif 3 (NUDT3), also known as nucleoside diphosphate-linked moiety X motif 3, is a member of the Nudix hydrolase family. These proteins are characterized by the presence of a conserved Nudix domain, which is involved in the hydrolysis of nucleoside diphosphates linked to other moieties, such as sugars, alcohols, or phosphates. NUDT3 plays a crucial role in maintaining cellular homeostasis by preventing the accumulation of potentially harmful nucleotide derivatives.

The NUDT3 gene is located on chromosome 6p21.31 and consists of at least four exons . The gene encodes a protein with a molecular mass of approximately 19.5 kDa, comprising 172 amino acids . The protein contains a Nudix domain with conserved glutamate residues that are essential for binding the Mg(2+) substrate complex and catalyzing the hydrolysis reaction .

NUDT3 is ubiquitously expressed in various tissues, with prominent expression in the brain, heart, pancreas, and liver . The protein functions as a diphosphoinositol polyphosphate phosphohydrolase, hydrolyzing diphosphate groups from diphosphoinositol pentakisphosphate (PP-InsP5) and bisdiphosphoinositol tetrakisphosphate ((PP)2-InsP4) . This activity is crucial for regulating the levels of these signaling molecules within the cell.

Recombinant human NUDT3 is produced using various expression systems, such as E. coli or HEK293T cells . The recombinant protein is often fused with tags, such as His or DDK, to facilitate purification through affinity chromatography . The purified recombinant protein retains its enzymatic activity and can be used for various research applications, including studying its biochemical properties and potential therapeutic uses.