NUDT14 Human

Nudix Type Motif 14 Human Recombinant
Cat. No.
BT16447
Source
Escherichia Coli.
Synonyms
UGPP, UGPPase, Uridine diphosphate glucose pyrophosphatase, UDPG pyrophosphatase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14, NUDT14.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NUDT14 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 245 amino acids (1-222) and having a molecular mass of 26.5kDa. NUDT14 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Uridine diphosphate glucose pyrophosphatase (NUDT14) belongs to the nudix hydrolase family. This cytoplasmic protein plays a crucial role as a sugar donor in various glycosylation processes, including glycogen synthesis. Its primary function involves hydrolyzing ADP-ribose into ribose 5-phosphate and AMP, as well as UDP-glucose to glucose 1-phosphate and UMP. NUDT14, encoded by a gene on human chromosome 14, functions as a homodimer and requires magnesium as a cofactor.
Description
Recombinant human NUDT14, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 245 amino acids (residues 1-222). With a molecular weight of 26.5 kDa, this protein features a 23 amino acid His-tag at the N-terminus and undergoes purification through proprietary chromatographic techniques.
Physical Appearance
The product is a clear, sterile-filtered solution.
Formulation
The NUDT14 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the protein at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing cycles should be avoided.
Purity
The purity of NUDT14 is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
UGPP, UGPPase, Uridine diphosphate glucose pyrophosphatase, UDPG pyrophosphatase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14, NUDT14.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ.

Product Science Overview

Structure and Expression

Human recombinant NUDT14 is typically produced in Escherichia coli (E. coli) as a non-glycosylated polypeptide chain. The recombinant protein often includes additional tags for purification purposes. For instance, NUDT14 can be expressed as an N-terminal GST-fusion protein or with a His-tag at the N-terminus . The molecular weight of the recombinant NUDT14 protein can vary depending on the tags used, but it generally ranges from 26.5 kDa to 50.9 kDa .

Function

NUDT14 specifically hydrolyzes ADP-ribose to AMP and ribose-5-phosphate. This activity is essential for maintaining cellular homeostasis by regulating the levels of ADP-ribose, which can be a byproduct of various metabolic processes. By controlling ADP-ribose levels, NUDT14 helps prevent the accumulation of potentially toxic intermediates that could disrupt cellular functions.

Biological Significance

The activity of NUDT14 is significant in various physiological processes, including:

  • DNA Repair: ADP-ribose is involved in the post-translational modification of proteins, particularly in the context of DNA repair. By hydrolyzing ADP-ribose, NUDT14 indirectly influences DNA repair mechanisms.
  • Signal Transduction: ADP-ribose can act as a signaling molecule. NUDT14 helps modulate signal transduction pathways by regulating ADP-ribose levels.
  • Metabolic Regulation: The hydrolysis of ADP-ribose by NUDT14 is part of broader metabolic pathways that ensure cellular energy balance and metabolic regulation.
Research and Applications

Recombinant NUDT14 is widely used in research to study its enzymatic properties, substrate specificity, and role in cellular metabolism. It is also utilized in structural biology to understand the molecular mechanisms underlying its function. The availability of recombinant NUDT14 allows for detailed biochemical and biophysical analyses, which can provide insights into its potential as a therapeutic target.

In summary, Nudix Type Motif 14 (Human Recombinant) is a vital enzyme with significant roles in cellular metabolism, DNA repair, and signal transduction. Its recombinant form, produced in E. coli, is a valuable tool for scientific research, enabling a deeper understanding of its functions and potential applications in medicine.

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