NUDT4 Human

Nudix Type Motif 4 Human Recombinant
Cat. No.
BT17769
Source
Escherichia Coli.
Synonyms
Diphosphoinositol polyphosphate phosphohydrolase 2, DIPP-2, EC 3.6.1.52, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 2, Nucleoside diphosphate-linked moiety X motif 4, NUDT4, DIPP2, KIAA0487, HDCMB47P, DIPP2beta, DIPP2alpha.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

NUDT4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (1-180 a.a) and having a molecular mass of 22.7kDa.
NUDT4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Nudix Type Motif 4 (NUDT4) is an enzyme that plays a crucial role in the regulation of diphosphoinositol polyphosphate levels. These high-energy molecules act as molecular switches, and their turnover by NUDT4 has significant regulatory implications, particularly in cellular processes like intracellular trafficking.
Description
This product consists of the recombinant NUDT4 protein, produced in E. coli. It is a single, non-glycosylated polypeptide chain with a molecular weight of 22.7 kDa. The protein sequence encompasses 203 amino acids, including a 23 amino acid His-tag fused at the N-terminus (amino acids 1-180 represent the NUDT4 sequence). The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
The product is a clear, colorless solution that has been sterilized through filtration.
Formulation
The NUDT4 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.1 M NaCl to ensure protein stability and integrity.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein like HSA or BSA to a final concentration of 0.1%. It is important to avoid repeated freezing and thawing of the product.
Purity
The purity of the NUDT4 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Diphosphoinositol polyphosphate phosphohydrolase 2, DIPP-2, EC 3.6.1.52, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 2, Nucleoside diphosphate-linked moiety X motif 4, NUDT4, DIPP2, KIAA0487, HDCMB47P, DIPP2beta, DIPP2alpha.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMKFKPN QTRTYDREGF KKRAACLCFR SEQEDEVLLV SSSRYPDQWI VPGGGMEPEE EPGGAAVREV YEEAGVKGKL GRLLGIFENQ DRKHRTYVYV LTVTEILEDW EDSVNIGRKR EWFKVEDAIK VLQCHKPVHA EYLEKLKLGC SPANGNSTVP SLPDNNALFV TAAQTSGLPS SVR.

Product Science Overview

Function and Importance

NUDT4 plays a crucial role in the regulation of intracellular levels of diphosphoinositol polyphosphates. These high-energy molecules are involved in various cellular processes, including signal transduction, energy metabolism, and regulation of intracellular trafficking. The turnover of these molecules by NUDT4 represents a molecular switching activity with significant regulatory consequences .

Structure and Expression

The recombinant human NUDT4 protein is typically expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques. The protein is often tagged with a His-tag at the N-terminus to facilitate purification. The recombinant protein corresponds to the amino acids 1-180 of the human NUDT4 sequence .

Applications

Recombinant NUDT4 is used in various research applications, including studies on intracellular signaling pathways, energy metabolism, and the regulation of intracellular trafficking. It is also used to investigate the molecular mechanisms underlying the regulation of diphosphoinositol polyphosphates .

Storage and Stability

Recombinant NUDT4 protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity. The protein is typically supplied in a buffer containing Tris-HCl, glycerol, and NaCl .

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