NUDT4 plays a crucial role in the regulation of intracellular levels of diphosphoinositol polyphosphates. These high-energy molecules are involved in various cellular processes, including signal transduction, energy metabolism, and regulation of intracellular trafficking. The turnover of these molecules by NUDT4 represents a molecular switching activity with significant regulatory consequences .
The recombinant human NUDT4 protein is typically expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques. The protein is often tagged with a His-tag at the N-terminus to facilitate purification. The recombinant protein corresponds to the amino acids 1-180 of the human NUDT4 sequence .
Recombinant NUDT4 is used in various research applications, including studies on intracellular signaling pathways, energy metabolism, and the regulation of intracellular trafficking. It is also used to investigate the molecular mechanisms underlying the regulation of diphosphoinositol polyphosphates .