NUCB2 Human, His
Nucleobindin-2 Human Recombinant, His Tag
Cat. No.
BT20290
Source
Escherichia Coli.
Synonyms
Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NUCB2, NEFA, Nesfatin.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
The Recombinant Human NUCB2 (Nesfatin) produced in E.coli has a molecular mass of 10.79kDa containing 92 amino acid residues of the human NUCB2 and fused to a 10 a.a. His tag at N-terminus.
Product Specs
Introduction
Nucleobindin-2, also known as NUCB2 or Nesfatin, is a member of the EF-hand calcium-binding protein family. This protein plays a role in calcium homeostasis and exhibits multifunctional properties, interacting with calcium ions (Ca2+), nucleic acids, and various regulatory proteins involved in different signaling pathways. NUCB2 (Nesfatin) is found within the neuronal cell bodies and dendrites of the mouse brain.
Description
Recombinant Human NUCB2 (Nesfatin) is produced in E. coli and has a molecular weight of 10.79 kDa. This recombinant protein comprises 92 amino acid residues of the human NUCB2 protein with a 10 amino acid Histidine tag fused at the N-terminus.
Formulation
NUCB2 (Nesfatin) is supplied as a lyophilized powder. The protein was initially filtered through a 0.4 μm filter and lyophilized from a solution of 20mM Tris buffer and 50mM NaCl at a pH of 7.5 with a protein concentration of 0.5 mg/ml.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve an approximate concentration of 0.5 mg/ml. Allow the pellet to dissolve completely. This product is not sterile. Before use in cell culture, filter the reconstituted protein through an appropriate sterile filter.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the protein solution to minimize repeated freezing and thawing cycles. The reconstituted protein remains stable at 4°C for a limited period and shows no significant change after two weeks at this temperature.
Applications
This product is suitable for use in Western blotting applications.
Synonyms
Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NUCB2, NEFA, Nesfatin.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL.
Product Science Overview
Gene and Protein Structure
The NUCB2 gene encodes the Nucleobindin-2 protein, which is a protein-coding gene. It is associated with several diseases, including diabetes mellitus and epilepsy . The protein has a molecular mass of approximately 10.79 kDa and contains 92 amino acid residues. The recombinant form of this protein, produced in Escherichia coli, is fused to a 10 amino acid His tag at the N-terminus .
Biological Functions
Nucleobindin-2 is involved in multiple biological functions:
- Calcium Homeostasis: It plays a crucial role in maintaining calcium levels within cells.
- Regulation of Food Intake: Nesfatin-1, a peptide derived from NUCB2, acts as an anorexigenic agent, regulating food intake and energy homeostasis in the hypothalamus in a leptin-independent manner .
- Blood Pressure Modulation: It may exert hypertensive effects and modulate blood pressure by acting on peripheral arterial resistance .
Expression and Localization
Recombinant Form and Applications
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