NUCB2 Human
Nucleobindin-2 Human Recombinant
Cat. No.
BT20153
Source
Escherichia Coli.
Synonyms
Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NUCB2, NEFA, Nesfatin.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
The Recombinant Human NUCB2 (Nesfatin) produced in E.coli has a molecular mass of 9.7kDa containing 82 amino acid residues of the human NUCB2.
Product Specs
Introduction
Nucleobindin-2, also called NUCB2 or Nesfatin, is a calcium-binding protein with an EF-hand motif. It plays a role in calcium regulation and exhibits diverse functions by interacting with calcium ions, nucleic acids, and various regulatory proteins within different signaling pathways. In mice, NUCB2 (Nesfatin) is found in neuronal cell bodies and dendrites throughout the brain.
Description
Recombinant Human NUCB2 (Nesfatin), produced in E. coli, is a 9.7 kDa protein comprising 82 amino acids of the human NUCB2 sequence.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The NUCB2 protein was lyophilized from a 0.2µm filtered solution at a concentration of 1mg/ml in 1x PBS, pH 7.4.
Solubility
To reconstitute lyophilized NUCB2, it is recommended to dissolve it in sterile 18M-cm H2O to a concentration of at least 100µg/ml. The solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized NUCB2 remains stable at room temperature for up to 3 weeks; however, for extended storage, it should be stored in a dry environment below -18°C. After reconstitution, NUCB2 can be stored at 4°C for 2 to 7 days. For long-term storage, freezing below -18°C is recommended. It is advisable to add a carrier protein like 0.1% HSA or BSA for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95% using the following methods:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Synonyms
Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NUCB2, NEFA, Nesfatin.
Source
Escherichia Coli.
Amino Acid Sequence
VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL.
Product Science Overview
Structure and Function
Nesfatin-1
Nesfatin-1, derived from the N-terminal region of NUCB2, is a leptin-independent peptide hormone with strong anorexigenic properties. It plays a crucial role in regulating energy homeostasis, appetite, and body weight . Nesfatin-1 is produced in the hypothalamus and other brain regions, as well as in peripheral tissues .
Nesfatin-2 and Nesfatin-3
Recombinant Human Nucleobindin-2
Recombinant human Nucleobindin-2 is produced using E. coli expression systems. It is a single non-glycosylated polypeptide chain containing 82 amino acids corresponding to nesfatin-1/Nucleobindin-2 . The recombinant protein is fully biologically active and is used in various research applications to study its role in metabolism, appetite regulation, and other physiological processes .
Applications and Storage
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