NT5E (CD73) Mouse

5’-Nucleotidase Ecto (CD73), also known as NT5E, is an enzyme that plays a crucial role in various physiological processes. It is a glycosyl phosphatidylinositol (GPI)-anchored membrane protein that catalyzes the hydrolysis of extracellular adenosine monophosphate (AMP) to adenosine . This enzyme is expressed on the surface of multiple cell types, including T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells . CD73 is involved in regulating immune responses, inflammation, and epithelial ion transport .

Recombinant mouse CD73 can be prepared using various expression systems. One common method involves using the baculovirus expression system to produce a soluble version of mouse NT5E (mNT5E) . The enzyme is then purified using affinity chromatography techniques. The purified recombinant mNT5E can be used in biochemical assays to study its activity and inhibition . Additionally, the enzyme can be conjugated with fluorescent markers for use in flow cytometric analysis .

CD73 catalyzes the conversion of AMP to adenosine, a reaction that plays a significant role in purinergic signaling . This enzymatic activity is crucial for maintaining immune system homeostasis by mediating the gradual hydrolysis of ATP and ADP to anti-inflammatory adenosine . The enzyme’s activity can be inhibited by specific inhibitors such as α,β-methylene-adenosine 5’-diphosphate (α,β-me-ADP), which selectively targets NT5E . The hydrolysis of AMP by CD73 is essential for regulating various physiological processes, including hypoxia, inflammation, and nociception .