CD73 Antibody
CD73, Mouse Anti Human
Cat. No.
BT1914
Source
Synonyms
5'-nucleotidase, Ecto-5'-nucleotidase, 5'-NT, CD73, NT5E, NT5, NTE, NT, eN, eNT, E5NT.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5-prime mononucleotides to nucleosides, with AMP being the preferred substrate at a neutral pH. CD73, a dimer composed of two identical 70kDa subunits, is anchored to the external surface of the plasma membrane via a glycosyl phosphatidyl inositol linkage. Serving as a marker for lymphocyte differentiation, CD73 deficiency is observed in various immunodeficiency disorders. Other forms of 5-prime nucleotidase, present in the cytoplasm and lysosomes, can be differentiated from ecto-NT5 based on substrate affinities, divalent magnesium ion requirement, ATP activation, and inorganic phosphate inhibition.
Physical Appearance
Colorless solution, sterile filtered.
Formulation
Concentration: 1mg/ml. Formulation: PBS (pH 7.4) with 0.1% Sodium Azide.
Storage Procedures
For storage up to 1 month, maintain at 4°C. For extended periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
CD73 antibody has undergone testing via ELISA and Western blot analysis to confirm its specificity and reactivity. However, due to variations in applications, it's recommended to titrate the reagent for each experiment to achieve optimal results. Suggested dilution range for Western blot analysis: 1:1,000 to 1:2,000. Initial dilution recommendation: 1:1,000.
Synonyms
5'-nucleotidase, Ecto-5'-nucleotidase, 5'-NT, CD73, NT5E, NT5, NTE, NT, eN, eNT, E5NT.
Purification Method
CD73 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P2B6AT.
Immunogen
Anti-human CD73 mAb , is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD73 amino acids 27-252 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Mouse Anti-Human CD73 Antibodies
Mouse anti-human CD73 antibodies are monoclonal antibodies derived from mice that are specifically designed to target and bind to human CD73. These antibodies are commonly used in research and clinical settings for various applications, including:
- Flow Cytometry: To identify and quantify CD73 expression on the surface of cells.
- Immunohistochemistry: To visualize CD73 expression in tissue samples.
- Functional Studies: To investigate the role of CD73 in various biological processes and disease states.
Recombinant mouse anti-human CD73 antibodies are produced using recombinant DNA technology, which allows for the generation of highly specific and consistent antibodies . These antibodies are valuable tools in both basic research and clinical diagnostics, providing insights into the role of CD73 in health and disease.
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