NT-proBNP Human
Escherichia Coli.
NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
NT-Pro-B-type Natriuretic Protein Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 76 amino acid and having a molecular mass of approximately 8.5kDa.
NT-proBNP is purified by proprietary chromatographic techniques.
Product Specs
Natriuretic Peptide Precursor B, also known as BNP, is a cardiac hormone with multiple biological functions. These include promoting the excretion of sodium (natriuresis), relaxing blood vessels (vasorelaxation), increasing urine production (diuresis), and suppressing the release of renin and aldosterone. BNP plays a crucial role in maintaining cardiovascular balance. Additionally, BNP contributes to restoring the body's equilibrium of salt and water, thereby enhancing heart function.
Recombinant Human NT-Pro-B-type Natriuretic Protein, produced in E. coli, is a polypeptide chain without any sugar molecules attached (non-glycosylated). It comprises 76 amino acids and has a molecular weight of about 8.5 kDa.
The purification of NT-proBNP is achieved through specific chromatographic methods.
Sterile Filtered White lyophilized (freeze-dried) powder.
The product is freeze-dried from a 0.2µm filtered solution concentrated in a buffer of 20mM Tris-HCl at pH 8.0 and 150mM NaCl.
For reconstitution of the lyophilized NT-Pro-B-type Natriuretic Protein, sterile 18MΩ-cm H2O is recommended. The initial concentration should be at least 100µg/ml. Further dilutions can be made in other aqueous solutions.
While the lyophilized NT-proBNP remains stable for 3 weeks at room temperature, it is best stored in a dry environment below -18°C. Once reconstituted, NT-Pro-B-type Natriuretic Protein should be kept at 4°C for a period of 2-7 days. For long-term storage, it should be kept frozen below -18°C.
It's important to avoid repeated freezing and thawing.
The purity is determined to be greater than 98.0% using the following methods:
(a) Analysis by RP-HPLC (Reverse Phase High-Performance Liquid Chromatography).
(b) Analysis by SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).
NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.
Escherichia Coli.
HPLGSPGSAS DLETSGLQEQ RNHLQGKLSE LQVEQTSLEP LQESPRPTGV WKSREVATEG IRGHRKMVLY TLRAPR.
Product Science Overview
NT-proBNP acts as a cardiac hormone with a variety of biological actions, including:
- Natriuresis: Promoting the excretion of sodium through urine.
- Vasorelaxation: Relaxing blood vessels to reduce blood pressure.
- Diuresis: Increasing urine production to help remove excess fluid from the body.
- Inhibition of Renin and Aldosterone Secretion: Helping to regulate blood pressure and fluid balance .
These actions collectively contribute to maintaining cardiovascular homeostasis, restoring the body’s salt and water balance, and improving heart function .
The lyophilized form of NT-proBNP is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term stability. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use. It is crucial to avoid freeze-thaw cycles to maintain the protein’s integrity .
