BNP (27-102) Human
NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.
Greater than 95.0% as determined by SDS-PAGE.
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Description
BNP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 27-102) containing 83 amino acids including a 7 a.a N-terminal His tag. The total molecular mass is 9.4kDa (calculated).
Product Specs
Natriuretic Peptide Precursor B, also known as BNP, is a cardiac hormone with various biological effects. These include promoting the excretion of sodium and water (natriuresis and diuresis), relaxing blood vessels (vasorelaxation), and inhibiting the production of renin and aldosterone. BNP is believed to be crucial for maintaining cardiovascular balance, restoring the body's salt and water equilibrium, and enhancing heart function.
Recombinant Human BNP, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 83 amino acids (a.a 27-102). It includes a 7 a.a N-terminal His tag and has a molecular weight of 9.4kDa (calculated).
The BNP product is filtered (0.4 µm) and lyophilized from a solution containing 0.5mg/ml BNP, 20 mM Tris buffer, and 50 mM NaCl at a pH of 7.5.
To create a working stock solution, add deionized water to the lyophilized pellet until it reaches a concentration of approximately 0.5mg/ml. Allow the pellet to fully dissolve. Please note that this BNP product is not sterile. Before using it in cell culture, filter it through a suitable sterile filter.
The purity of this product is greater than 95.0% as determined by SDS-PAGE analysis.
NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.
MKHHHHHHPL GSPGSASDLE TSGLQEQRNH LQGKLSELQV EQTSLEPLQE SPRPTGVWKS REVATEGIRG HRKMVLYTLR APR.
Product Science Overview
B-type Natriuretic Protein (BNP), also known as B-type Natriuretic Peptide, is a hormone produced by the heart. It plays a crucial role in cardiovascular homeostasis by regulating blood pressure, fluid balance, and electrolyte levels. The specific form, B-type Natriuretic Protein (27-102 a.a.) (Human Recombinant), refers to a recombinant version of the protein that includes amino acids 27 to 102 of the human BNP sequence.
BNP was first discovered in brain tissue, which is why it is sometimes referred to as “brain” natriuretic peptide . However, it is predominantly produced by the ventricular myocardium of the heart . The protein is initially synthesized as a preprohormone, which is then cleaved to form the active hormone. The active form of BNP consists of 32 amino acids, but the recombinant version often includes additional amino acids for stability and activity.
BNP has several important biological functions:
- Natriuresis: It promotes the excretion of sodium through urine, helping to reduce blood volume and pressure.
- Diuresis: It increases urine production, aiding in the removal of excess fluid from the body.
- Vasorelaxation: It causes blood vessels to relax, which lowers blood pressure.
- Inhibition of Renin and Aldosterone Secretion: It helps to regulate the renin-angiotensin-aldosterone system, which is involved in blood pressure control and fluid balance .
BNP levels are commonly measured in clinical settings to diagnose and manage heart failure. Elevated levels of BNP in the blood are indicative of heart failure, as the heart releases more BNP in response to increased pressure and volume load . The BNP test is a valuable tool for distinguishing between heart failure and other conditions that cause similar symptoms, such as pneumonia .
Recombinant BNP, such as B-type Natriuretic Protein (27-102 a.a.) (Human Recombinant), is produced using recombinant DNA technology. This involves inserting the gene encoding BNP into a host organism, such as bacteria or yeast, which then produces the protein. Recombinant BNP is used in research and therapeutic applications, including the treatment of acute heart failure.
