MIP 1a Rat
Macrophage Inflammatory Protein-1 Alpha Rat Recombinant (CCL3)
Cat. No.
BT20392
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Macrophage Inflammatory Protein-1 alpha Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton.
The MIP-1a is purified by proprietary chromatographic techniques.
The MIP-1a is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Macrophage Inflammatory Proteins (MIPs) are chemokines, a family of chemotactic cytokines. Humans possess two primary forms: MIP-1a (officially CCL3) and MIP-1b (officially CCL4). Macrophages produce significant quantities of both MIP-1a and MIP-1b upon stimulation by bacterial endotoxins. These proteins activate human granulocytes, including neutrophils, eosinophils, and basophils, potentially causing acute neutrophilic inflammation. Furthermore, they stimulate fibroblasts and macrophages to synthesize and release additional pro-inflammatory cytokines like interleukin 1 (IL-1), IL-6, and TNF-a. The genes encoding CCL3 and CCL4 are both situated on human chromosome 17.
Description
Recombinant Rat Macrophage Inflammatory Protein-1 alpha, produced in E. coli, is a single-chain, non-glycosylated polypeptide. It consists of 69 amino acids, resulting in a molecular weight of 7853 Daltons. The purification of MIP-1a is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein solution, devoid of any additives and at a concentration of 1 mg/ml, undergoes lyophilization.
Solubility
To reconstitute the lyophilized Macrophage Inflammatory Protein-1a, it is advised to dissolve it in sterile 18MΩ-cm H2O to a minimum concentration of 100µg/ml. This solution can be further diluted using other aqueous solutions as needed.
Stability
Lyophilized MIP-1a demonstrates stability at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it in a desiccated state below -18°C. After reconstitution, CCL3 should be stored at 4°C for a period of 2-7 days. For long-term storage, adding a carrier protein like 0.1% HSA or BSA is advisable. It is crucial to avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% through the following analyses: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The biological activity is assessed based on the protein's ability to chemoattract rat peritoneal macrophages. Using a concentration of 60 ng/ml, the specific activity is determined to be 16,667 IU/mg.
Synonyms
Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Tyr-Gly-Ala.
Product Science Overview
Discovery and Structure
Biological Functions
MIP-1α/CCL3 is involved in several biological processes:
- Chemotaxis: It recruits inflammatory cells such as macrophages, lymphocytes, and eosinophils to sites of inflammation .
- Wound Healing: It plays a role in the wound healing process by attracting cells necessary for tissue repair .
- Bone Resorption: MIP-1α/CCL3 activates osteoclasts, leading to bone resorption and destruction .
- Immune Response: It maintains the effector immune response and inhibits stem cell proliferation .
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