MIP 1b Mouse
Macrophage Inflammatory Protein-1 beta Mouse Recombinant (CCL4)
Cat. No.
BT20590
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A4, CCL4, Macrophage inflammatory protein 1-beta, MIP-1- beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, H400, SIS-gamma, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, chemokine (C-C motif) ligand 4, ACT2, G-26, LAG1, MIP1B, SCYA2, SCYA4, AT744.1, MGC104418, MGC126025, MGC126026.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Macrophage Inflammatory Protein-1 beta Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7808 Dalton.
The MIP-1b is purified by proprietary chromatographic techniques.
The MIP-1b is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Macrophage Inflammatory Proteins (MIPs), classified as chemokines, are chemotactic cytokines. Humans possess two primary forms: MIP-1α (officially CCL3) and MIP-1β (officially CCL4). Macrophages primarily produce both forms upon stimulation by bacterial endotoxins. These proteins activate human granulocytes, including neutrophils, eosinophils, and basophils, potentially resulting in acute neutrophilic inflammation. Furthermore, they stimulate fibroblasts and macrophages to synthesize and release other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6, and TNF-α. Notably, the genes encoding CCL3 and CCL4 are both situated on human chromosome 17.
Description
Recombinant Mouse Macrophage Inflammatory Protein-1 beta, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 69 amino acids. With a molecular weight of 7808 Daltons, MIP-1b undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 1 mg/ml solution in water, free of additives.
Solubility
Reconstitute the lyophilized Mouse MIP-1b in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Mouse MIP-1b remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, store Mouse CCL4 at 4°C for 2-7 days. For long-term storage, freeze at -18°C. To enhance stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 98.0% as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis, and (b) SDS-PAGE analysis.
Biological Activity
The biological activity of Mouse MIP-1 beta is determined by its chemoattractant properties on human blood monocytes. At concentrations of 20-100 ng/ml, it exhibits a specific activity ranging from 10,000 to 50,000 IU/mg.
Synonyms
Small inducible cytokine A4, CCL4, Macrophage inflammatory protein 1-beta, MIP-1- beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, H400, SIS-gamma, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, chemokine (C-C motif) ligand 4, ACT2, G-26, LAG1, MIP1B, SCYA2, SCYA4, AT744.1, MGC104418, MGC126025, MGC126026.
Source
Escherichia Coli.
Amino Acid Sequence
APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA.
Product Science Overview
Structure and Source
Function
CCL4 is involved in several key functions:
- Chemotaxis: It attracts immune cells such as monocytes, natural killer (NK) cells, and T cells to sites of infection or inflammation .
- Proinflammatory Effects: It induces the release of proinflammatory cytokines, mast cell degranulation, and NK cell activation .
- HIV Suppression: CCL4, along with CCL3 (MIP-1α) and RANTES, has been shown to be a major HIV-suppressive factor. It interacts with the CCR5 receptor on CD4+ T cells, which is also a major receptor for HIV entry into these cells .
Applications in Research
Recombinant mouse CCL4 is widely used in immunology and stem cell biology research. It is utilized to study:
- Immune Cell Migration: Researchers use CCL4 to investigate the mechanisms of immune cell migration and chemotaxis .
- Inflammatory Responses: It helps in understanding the pathways involved in inflammation and the role of chemokines in these processes .
- HIV Research: Due to its role in HIV suppression, CCL4 is used in studies aimed at developing new therapeutic strategies against HIV .
Production and Purity
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
