MIP 1a Human

Macrophage Inflammatory Protein-1 Alpha Human Recombinant (CCL3)
Cat. No.
BT19984
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Macrophage Inflammatory Protein-1 alpha Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Dalton.
The MIP-1a is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Macrophage Inflammatory Proteins (MIPs), chemokines, are crucial in immune responses. Humans possess two primary forms: MIP-1a (CCL3) and MIP-1b (CCL4). Produced by macrophages upon bacterial endotoxin stimulation, they activate granulocytes (neutrophils, eosinophils, basophils), potentially causing acute neutrophilic inflammation. Furthermore, they stimulate the production of pro-inflammatory cytokines like interleukin 1 (IL-1), IL-6, and TNF-a from fibroblasts and macrophages. Notably, the genes encoding CCL3 and CCL4 reside on human chromosome 17.
Description
Recombinant Human Macrophage Inflammatory Protein-1 alpha, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 70 amino acids with a molecular weight of 7820 Daltons. The purification of MIP-1a is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein solution, at a concentration of 0.55 mg/ml and without any additives, was lyophilized.
Solubility
For reconstitution, it is advised to dissolve the lyophilized Macrophage Inflammatory Protein-1a in sterile 18MΩ-cm H2O at a minimum concentration of 100µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
While lyophilized MIP-1a remains stable at room temperature for up to 3 weeks, it is recommended to store it desiccated below -18°C. After reconstitution, CCL3 should be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is advised. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined using the following methods, and is greater than 98.0%: (a) RP-HPLC analysis (b) SDS-PAGE analysis.
Biological Activity
The biological activity is assessed based on the chemo-attraction of human monocytes, using a concentration range of 1-10ng/ml. This corresponds to a Specific Activity of 100,000-1,000,000 IU/mg.
Synonyms
Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Leu-Ala-Ala.

Product Science Overview

Introduction

Macrophage Inflammatory Protein-1 Alpha (MIP-1α), also known as CCL3, is a chemokine that plays a crucial role in the immune response. It is produced by macrophages and other cell types and is involved in the recruitment and activation of various immune cells. This article delves into the background, biological functions, and clinical significance of MIP-1α/CCL3.

Discovery and Nomenclature

MIP-1α was first discovered by Stephen D. Wolpe in 1988 . It belongs to the CC subfamily of chemokines, which are characterized by two adjacent cysteine residues near their amino terminus. The official name for MIP-1α is CCL3, and it is also known by several other names, including G0/G1 switch regulatory protein 19-1, PAT 464.1, and small-inducible cytokine A3 .

Biological Functions

MIP-1α/CCL3 is a multifunctional peptide that performs various biological functions:

  • Chemotaxis: It recruits macrophages, lymphocytes, and eosinophils to sites of inflammation via the CCR1 or CCR5 receptors .
  • Bone Resorption: It activates bone resorption cells and directly induces bone destruction, playing a significant role in bone remodeling by inducing osteoclastogenesis .
  • Inflammatory Response: It induces inflammatory responses, including superoxide production by neutrophils .
  • Antiviral Activity: MIP-1α has been shown to have antiviral activity against HSV-1 and exhibits strong HIV-suppressive activity .
Clinical Significance

MIP-1α/CCL3 is associated with various inflammatory diseases and conditions that exhibit bone resorption, such as:

  • Periodontitis: Elevated levels of MIP-1α/CCL3 are found in the biological fluids of patients with periodontitis .
  • Multiple Myeloma: It plays a role in the pathogenesis of multiple myeloma by recruiting inflammatory cells and promoting osteoclastogenesis .
  • Rheumatoid Arthritis: MIP-1α/CCL3 is involved in the inflammatory processes of rheumatoid arthritis .
  • Sjögren Syndrome: Increased concentrations of MIP-1α/CCL3 are detected in patients with Sjögren syndrome .
Recombinant Human MIP-1α/CCL3

Recombinant human MIP-1α/CCL3 is a human full-length protein expressed in Escherichia coli with high purity and low endotoxin levels . It is used in various research applications, including SDS-PAGE, functional assays, and HPLC . The recombinant protein retains its biological activity and is used to study the chemotactic and inflammatory properties of MIP-1α/CCL3 .

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