MIP 1a Human, His
Greater than 90.0% as determined by SDS-PAGE.
Description
MIP-1a Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 90 amino acids (24-92 a.a.) and having a molecular mass of 10.0kDa. MIP-1a is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Recombinant Mouse MIP-1a, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 90 amino acids (specifically, amino acids 24 to 92) and has a molecular weight of 10.0 kDa. This MIP-1a protein has a 21 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
The MIP-1a protein solution has a concentration of 0.25 mg/ml. It is prepared in a buffer consisting of 10 mM Sodium Citrate (pH 3.5) and 20% glycerol.
For short-term storage (up to 2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
The purity is determined to be greater than 90.0% using SDS-PAGE analysis.
MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQVCADPSEEWVQ KYVSDLELSA.
Product Science Overview
Macrophage Inflammatory Protein-1 Alpha (MIP-1α), also known as CCL3, is a chemotactic cytokine secreted by macrophages. It plays a crucial role in the immune response by recruiting inflammatory cells to sites of infection or injury . The recombinant form of this protein, tagged with a His (histidine) tag, is used in various research and clinical applications.
MIP-1α/CCL3 belongs to the CC subfamily of chemokines, which are characterized by two adjacent cysteine residues near their amino terminus . The His tag is a sequence of histidine residues added to the protein to facilitate purification and detection.
MIP-1α/CCL3 binds to specific receptors on the surface of target cells, including CCR1, CCR4, and CCR5 . This binding triggers a cascade of intracellular signals that lead to the recruitment of immune cells such as macrophages, lymphocytes, and eosinophils . Additionally, MIP-1α/CCL3 has been shown to inhibit the proliferation of hematopoietic stem cells and induce bone resorption by activating osteoclasts .
MIP-1α/CCL3 is involved in various physiological and pathological processes. It plays a significant role in:
- Inflammatory Responses: By recruiting immune cells to sites of infection or injury, MIP-1α/CCL3 helps to initiate and sustain inflammatory responses .
- Bone Resorption: MIP-1α/CCL3 activates osteoclasts, leading to bone resorption. This is particularly relevant in diseases such as periodontitis and multiple myeloma .
- HIV Suppression: MIP-1α/CCL3 has been identified as one of the major HIV-suppressive factors produced by CD8+ T-cells. It inhibits the replication of various strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) .
