MIP 1a Mouse, His

Macrophage Inflammatory Protein-1 Alpha Mouse Recombinant (CCL3), His Tag
Cat. No.
BT20294
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA, TY-5, SIS-alpha, Heparin-binding chemotaxis protein, L2G25B.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MIP-1a Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 93 amino acids (24-92 a.a.) and having a molecular mass of 10.4kDa.
MIP-1a is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Macrophage Inflammatory Proteins (MIPs) are chemokines that play a crucial role in immune responses. In humans, MIP-1a (CCL3) and MIP-1b (CCL4) are produced by activated macrophages and contribute to inflammation by recruiting and activating granulocytes. These proteins also stimulate the production of other inflammatory mediators like IL-1, IL-6, and TNF-a. The genes encoding CCL3 and CCL4 are located on chromosome 17.
Description
Recombinant Mouse MIP-1a is produced in E. coli and consists of a single, non-glycosylated polypeptide chain of 93 amino acids (24-92 a.a.). This protein has a molecular mass of 10.4 kDa. A 24 amino acid His-tag is fused to the N-terminus to facilitate purification using chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The MIP-1a protein is supplied at a concentration of 0.5 mg/ml in a solution containing 20 mM Sodium citrate buffer (pH 3.5) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For long-term storage, freeze the protein at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freezing and thawing.
Purity
Purity is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA, TY-5, SIS-alpha, Heparin-binding chemotaxis protein, L2G25B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPYGAD TPTACCFSYS RKIPRQFIVD YFETSSLCSQ PGVIFLTKRN RQICADSKET WVQEYITDLE LNA.

Product Science Overview

Introduction

Macrophage Inflammatory Protein-1 Alpha (MIP-1α), also known as CCL3, is a chemokine that plays a crucial role in the immune system. It is involved in the recruitment and activation of various immune cells, including macrophages, lymphocytes, and eosinophils. The recombinant form of this protein, specifically the mouse version with a His tag, is widely used in research to study its functions and potential therapeutic applications.

Discovery and Structure

MIP-1α/CCL3 was first discovered by Stephen D. Wolpe in 1988 . It belongs to the CC subfamily of chemokines, characterized by two adjacent cysteine residues near their amino terminus. The recombinant mouse version of MIP-1α/CCL3 is expressed in HEK 293 cells and includes a His tag for purification purposes . This protein has a molecular weight of approximately 10.07 kDa .

Biological Functions

MIP-1α/CCL3 is a multifunctional peptide secreted by various hematopoietic and non-hematopoietic cells upon stimulation . Its primary functions include:

  • Recruitment of Inflammatory Cells: MIP-1α/CCL3 attracts macrophages, lymphocytes, and eosinophils to sites of inflammation via the CCR1 or CCR5 receptors .
  • Bone Resorption: It activates bone resorption cells and directly induces bone destruction, playing a significant role in bone remodeling processes .
  • Wound Healing: MIP-1α/CCL3 is involved in wound healing by recruiting cells necessary for tissue repair .
  • Inhibition of Stem Cells: It inhibits the proliferation of hematopoietic stem cells .
  • HIV Suppression: MIP-1α/CCL3 exhibits strong HIV-suppressive activity .
Clinical Significance

MIP-1α/CCL3 is associated with various inflammatory diseases and conditions that exhibit bone resorption, such as periodontitis, multiple myeloma, Sjögren syndrome, and rheumatoid arthritis . Elevated levels of MIP-1α/CCL3 can be detected in biological fluids of patients suffering from these diseases, indicating its potential as a diagnostic biomarker .

Recombinant Mouse MIP-1α/CCL3 (His Tag)

The recombinant mouse MIP-1α/CCL3 protein with a His tag is a full-length protein expressed in HEK 293 cells. It has a purity of ≥95% and an endotoxin level of ≤1.63 EU/mg . This recombinant protein is used in various applications, including HPLC and mass spectrometry (MS), to study its biological functions and interactions .

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